Description

H-D-Ala-Amc Tfa is a specialized, high-purity fluorogenic peptide substrate used extensively in biochemical research and pharmaceutical development. This compound is critical for studying enzyme kinetics and activity, particularly for proteases like calpain, due to its cleavable amide bond that releases a fluorescent signal. It is an essential tool for researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical manufacturing sectors seeking reliable and sensitive assay components.

Application

• Protease Activity Assays: Primary substrate for fluorometric assays to measure the activity of specific proteolytic enzymes, including calpain and other cysteine proteases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential enzyme inhibitors for therapeutic development.
• Biochemical Research: A key reagent for fundamental research into enzyme mechanisms, substrate specificity, and kinetic parameter determination.
• Diagnostic Kit Development: Incorporated into the development of in-vitro diagnostic (IVD) kits for detecting specific enzyme-related disease markers.
• Quality Control in Biologics: Employed in QC labs to monitor protease activity during the production and purification of biopharmaceuticals.
• Cell Biology Studies: Used in live-cell imaging and assays to visualize and quantify protease activity within cellular environments.

Basic Information

Product Name	H-D-Ala-Amc Tfa
CAS No.	201847-52-1
Molecular Formula	C₁₅H₁₈F₃N₃O₅
Molecular Weight	377.32 g/mol
Synonyms	H-D-Ala-AMC TFA; D-Alanine-7-amido-4-methylcoumarin Trifluoroacetate; (R)-2-((7-Amino-4-methyl-2-oxo-2H-chromen-3-yl)amino)propanoic acid trifluoroacetate; Calpain Substrate I, Fluorogenic; D-Ala-AMC TFA Salt; Suc-LLVY-AMC related substrate; Protease Fluorogenic Substrate; CAS 201847-52-1
EINECS	Contact for details

Quality Control

Our H-D-Ala-Amc Tfa is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and other critical parameters to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	≥ 95.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional in standard calpain assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.