Description

Cathepsin B Substrate I, Colorimetric is a specialized chromogenic peptide substrate designed for the selective detection and measurement of cathepsin B enzyme activity. This compound matters as a critical research tool for studying protease function in various biological pathways, particularly those related to cancer metastasis, inflammation, and neurodegenerative diseases. It is needed by researchers and scientists in pharmaceutical R&D, academic biochemistry laboratories, and diagnostic kit manufacturers for in vitro enzymatic assays and high-throughput screening applications.

Application

• Biochemical Research: Used as a selective substrate for kinetic studies and characterization of cathepsin B protease activity in purified enzyme preparations.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) assays to identify and evaluate potential cathepsin B inhibitors for therapeutic development in oncology and inflammatory diseases.
• Cell Biology Studies: Applied in cell-based assays to monitor intracellular cathepsin B activity, which is a biomarker for processes like apoptosis, tumor invasion, and lysosomal function.
• Diagnostic Development: Serves as a key component in developing colorimetric diagnostic kits for measuring cathepsin B levels in biological samples.
• Enzyme Mechanism Analysis: Utilized to study enzyme specificity, catalytic efficiency, and the biochemical mechanisms of cysteine proteases.
• Quality Control in Biomanufacturing: Can be used to test for the presence of specific protease activities during the purification and formulation of biopharmaceuticals.

Basic Information

Item	Detail
Product Name	Cathepsin B Substrate I, Colorimetric
CAS No.	201807-90-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Z-Arg-Arg-AMC; Z-Arg-Arg-7-amido-4-methylcoumarin; Benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin; Cathepsin B Fluorogenic Substrate; Cathepsin B Substrate (Z-RR-AMC); Cbz-Arg-Arg-AMC; Z-RR-AMC; CBZ-Arg-Arg-AMC
EINECS	Contact for details

Quality Control

Our Cathepsin B Substrate I, Colorimetric is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including purity assessment by HPLC and functional validation in enzymatic assays. Certificates of Analysis (COA) detailing identity, purity, and performance specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms to reference standard in cathepsin B assay
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.