Description

Boc-L-Leu-OH-1-13C Monohydrate is a stable isotope-labeled derivative of the amino acid L-Leucine, specifically enriched with carbon-13 at the 1-position. This compound is essential for advanced research and development, providing a critical tool for metabolic pathway tracing, protein structure elucidation, and quantitative proteomics using mass spectrometry. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic developers requiring high-purity, isotopically labeled building blocks for peptide synthesis and biomarker studies.

Application

• Isotope-Labeled Internal Standards for accurate quantification in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) bioanalysis.
• Metabolic Flux Analysis and tracer studies to investigate cellular metabolism, nutrient utilization, and disease mechanisms.
• Peptide & Protein Research as a labeled precursor for the solid-phase synthesis of peptides used in NMR spectroscopy and structural biology.
• Pharmaceutical Development for creating isotopically labeled drug candidates to study Absorption, Distribution, Metabolism, and Excretion (ADME) profiles.
• Diagnostic Reagent Manufacturing for use in high-sensitivity clinical assays and kits.
• Nutritional Science Research to trace amino acid metabolism and protein turnover in vivo.

Basic Information

Item	Details
Product Name	Boc-L-Leu-OH-1-13C Monohydrate
CAS No.	201740-80-9
Molecular Formula	C11H2113CNO5 • H2O
Molecular Weight	279.30 g/mol (for 13C1 isotopologue)
Synonyms	(2S)-2-[(tert-Butoxycarbonyl)amino]-4-methyl-1-(13C)pentanoic acid monohydrate; N-α-(tert-Butoxycarbonyl)-L-leucine-1-13C monohydrate; Boc-Leu-1-13C-OH • H2O; N-Boc-L-leucine-1-13C monohydrate; tert-Butoxycarbonyl-L-leucine-1-13C monohydrate; Boc-L-leucine-1-13C; BOC-Leu-1-13C; (S)-2-((tert-Butoxycarbonyl)amino)-4-methyl-1-(13C)pentanoic acid hydrate
EINECS	Contact for details

Quality Control

Every batch of Boc-L-Leu-OH-1-13C Monohydrate is manufactured and tested under strict quality management systems. We guarantee a minimum isotopic purity of 99 atom % 13C and a high chemical purity, verified by advanced analytical techniques including NMR, HPLC, and mass spectrometry. Certificates of Analysis (COA) detailing isotopic enrichment, chemical purity, and specific tests are provided with each shipment to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (13C)	≥ 99 atom %
Chemical Purity (HPLC)	≥ 98.0%
Specific Rotation [α]D20	-25° to -30° (c=1 in Acetic Acid)
Water Content (KF)	4.0% - 7.0% (Theoretical for monohydrate: ~6.45%)
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.