Description

n-(tert-Butoxycarbonyl)-L-Alanine-1-13C CAS NO 201740-78-5 is a stable isotope-labeled derivative of the amino acid L-alanine, where the carbon at the 1-position is enriched with the non-radioactive carbon-13 isotope. This compound is a critical building block for the synthesis of isotopically labeled peptides and proteins, enabling advanced research in metabolic studies, protein structure elucidation via NMR spectroscopy, and pharmacokinetic analysis. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs (Contract Research Organizations) specializing in proteomics and drug discovery.

Application

• Isotope-Labeled Peptide Synthesis: Serves as a key protected amino acid precursor for the solid-phase or solution-phase synthesis of carbon-13 labeled peptides for metabolic tracing and structural biology.
• NMR Spectroscopy Studies: Used to incorporate a site-specific 13C label into proteins, facilitating detailed conformational and dynamic analysis through Nuclear Magnetic Resonance (NMR).
• Mass Spectrometry Standards: Acts as an internal standard in quantitative LC-MS/MS assays for accurate measurement of alanine or alanine-containing compounds in biological matrices.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in the synthesis of isotopically labeled drug candidates to study absorption, distribution, metabolism, and excretion (ADME) profiles.
• Proteomics Research: Essential for metabolic labeling strategies (e.g., SILAC - Stable Isotope Labeling by Amino acids in Cell culture) to perform comparative quantitative proteomics.
• Biochemical Pathway Analysis: Used as a tracer to investigate specific metabolic pathways involving alanine, such as gluconeogenesis or amino acid transamination.

Basic Information

Product Name	n-(tert-Butoxycarbonyl)-L-Alanine-1-13C
CAS No.	201740-78-5
Molecular Formula	C8H1513CNO4
Molecular Weight	190.20 g/mol (for 13C1 isotopologue)
Synonyms	Boc-L-Alanine-1-13C; (2S)-2-[(tert-Butoxycarbonyl)amino]propanoic-1-13C acid; N-Boc-L-alanine-1-13C; N-(tert-Butoxycarbonyl)-L-alanine-1-13C; L-Alanine-1-13C, N-Boc-protected; Boc-Ala-1-13C-OH; tert-Butyl (2S)-2-amino-1-(13C)carboxyethylcarbamate; 1-13C-Boc-L-Ala
EINECS	Contact for details

Quality Control

Our n-(tert-Butoxycarbonyl)-L-Alanine-1-13C is manufactured under strict quality systems to ensure high isotopic and chemical purity, critical for sensitive research applications. Each batch is characterized by NMR, HPLC, and Mass Spectrometry to confirm identity, isotopic enrichment (typically >99 atom % 13C), and chemical purity (often >98%). Certificates of Analysis (COA) detailing these specifications are provided with every shipment. We adhere to relevant standards for research chemicals and can support custom synthesis to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Optical Rotation	[α]D20: -25° to -30° (c=1 in CHCl3)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.