Description

H-Ala-Ala-Pro-Leu-Pna Hcl is a synthetic peptide substrate, specifically a chromogenic p-nitroanilide (pNA) derivative, widely used in biochemical and pharmaceutical research. This compound is a critical tool for studying enzyme kinetics and activity, particularly for serine proteases and other hydrolytic enzymes. It is essential for researchers and manufacturers in the fields of proteomics, drug discovery, and diagnostic assay development who require high-purity, reliable substrates for accurate experimental results.

Application

• Enzyme Substrate for Protease Research: Used as a specific chromogenic substrate to measure the activity and kinetics of various proteolytic enzymes, including elastase and related serine proteases.
• Drug Discovery & High-Throughput Screening (HTS): Employed in biochemical assays to identify and characterize potential enzyme inhibitors for therapeutic development.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for detecting specific enzyme activities related to disease states.
• Biochemical Mechanism Studies: Facilitates research into enzyme specificity, catalytic mechanisms, and substrate binding interactions.
• Quality Control in Biopharma: Used in in-process testing and final product release assays to ensure the purity and activity of enzyme-based pharmaceuticals.

Basic Information

Product Name	H-Ala-Ala-Pro-Leu-Pna Hcl
CAS No.	201732-32-3
Molecular Formula	C24H36N6O6 • HCl
Molecular Weight	533.05 g/mol
Synonyms	Ala-Ala-Pro-Leu-pNA • HCl; AAPL-pNA; H-AAPL-pNA hydrochloride; L-Alanyl-L-alanyl-L-prolyl-L-leucine 4-nitroanilide hydrochloride; Substrate for Human Leukocyte Elastase (HLE); Peptide substrate 201732-32-3; AAPL p-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Ala-Ala-Pro-Leu-Pna Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and specific optical rotation verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production standards. Custom purity grades and bulk specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -15°C to -25°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.