Description

4-Nitrophenyl β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection and quantification of β-galactosidase enzyme activity. This compound matters for its critical role in biochemical research, diagnostics, and molecular biology, where precise enzymatic measurement is essential. It is primarily needed by professionals in the life sciences, pharmaceutical R&D, and clinical diagnostics sectors for applications in reporter gene assays, enzyme-linked immunosorbent assays (ELISA), and microbiological testing.

Application

• Enzymatic Assays: As a chromogenic substrate for β-galactosidase in reporter gene systems (e.g., lacZ) for monitoring gene expression.
• Diagnostic Kits: Used in clinical and research ELISA kits for the detection of specific antibodies, antigens, or pathogens.
• Microbiology: Employed in microbial identification and characterization, particularly for differentiating bacterial species based on β-galactosidase activity.
• Biochemical Research: A key reagent for studying enzyme kinetics, inhibition, and purification processes of β-galactosidase.
• Cell Biology: Utilized in histochemical staining to visualize β-galactosidase activity in tissues and cells.
• High-Throughput Screening (HTS): Applied in pharmaceutical discovery for screening compound libraries against β-galactosidase-related targets.
• Food & Beverage Testing: Potential use in assays for detecting microbial contamination or specific enzymatic activity in quality control.
• Academic & Teaching Laboratories: A standard reagent for practical experiments in biochemistry and molecular biology courses.

Basic Information

Product Name	4-Nitrophenyl β-D-Galactopyranoside
CAS No.	200422-18-0
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-D-galactopyranoside; PNPG; 4-Nitrophenyl β-D-galactoside; p-Nitrophenyl β-D-galactoside; β-Galactosidase substrate; 4-NPG; O-4-Nitrophenyl-β-D-galactopyranoside; 1-O-(4-Nitrophenyl)-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.