Description

p-Nitrophenyl 3,4-o-Isopropylidene-α-D-Galactopyranoside is a specialized glycoside derivative used as a critical chromogenic substrate in enzymatic assays. This compound is essential for researchers and manufacturers requiring precise, colorimetric detection of specific glycosidase enzyme activity. It is primarily utilized in the biochemical, pharmaceutical, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and quality control of enzymatic processes.

Application

• Enzymatic Substrate for α-Galactosidase: A key chromogenic substrate for the detection and quantification of α-galactosidase activity in research and diagnostic assays.
• Biochemical Research: Used in enzyme kinetics studies, mechanism of action investigations, and high-throughput screening for enzyme inhibitors.
• Diagnostic Reagent Manufacturing: Incorporated into kits for the diagnosis of genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Pharmaceutical Development: Employed in quality control and potency testing of enzyme replacement therapies and related biopharmaceuticals.
• Microbiology and Cell Biology: Used as a tool to study glycosidase function in various microorganisms and cellular pathways.

Basic Information

Product Name	p-Nitrophenyl 3,4-o-Isopropylidene-α-D-Galactopyranoside
CAS No.	29781-32-6
Molecular Formula	C₁₅H₁₉NO₈
Molecular Weight	341.32 g/mol
Synonyms	4-Nitrophenyl 3,4-O-isopropylidene-α-D-galactopyranoside; p-Nitrophenyl 3,4-O-(1-methylethylidene)-α-D-galactopyranoside; α-D-Galactopyranoside, 4-nitrophenyl 3,4-O-(1-methylethylidene)-; PNPGal-IP; 3,4-O-Isopropylidene-α-D-galactopyranosyl p-nitrophenyl ether; pNP-α-D-Gal-IP; Chromogenic α-galactosidase substrate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 3,4-o-Isopropylidene-α-D-Galactopyranoside is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and specific optical rotation measurement. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.