Description

4α,14-Dimethyl-9β,19-Cyclo-(25-14C)5α-Cholest-24-En-3β-Ol is a high-purity, carbon-14 labeled sterol derivative, specifically designed for advanced research and analytical applications. This compound is critical for metabolic and biosynthetic pathway tracing studies, offering unparalleled precision in tracking sterol transformations. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors requiring labeled standards for mass spectrometry, NMR, and other sophisticated analytical techniques.

Application

• Isotopic Tracer in Metabolic Studies: Used as a labeled precursor to investigate cholesterol biosynthesis and sterol metabolism pathways in biological systems.
• Internal Standard for Quantitative Analysis: Serves as a critical internal standard in LC-MS and GC-MS methods for the accurate quantification of sterols and related metabolites.
• Pharmaceutical Research & Development: Employed in drug discovery programs targeting enzymes in the sterol biosynthesis pathway, such as those involved in cholesterol-lowering therapies.
• Biochemical Research: Utilized in enzymatic assays and in vitro studies to elucidate the mechanism of action of sterol-modifying enzymes.
• Reference Material for Analytical Laboratories: Provides a certified reference material for method development, validation, and quality control in analytical testing.

Basic Information

Item	Details
Product Name	4α,14-Dimethyl-9β,19-Cyclo-(25-14C)5α-Cholest-24-En-3β-Ol
CAS No.	28288-92-8
Molecular Formula	C₂₉H₄₈O (with 14C at position 25)
Molecular Weight	~412.70 g/mol (exact mass depends on 14C enrichment)
Synonyms	(25-14C)-4α,14-Dimethyl-9β,19-cyclo-5α-cholest-24-en-3β-ol; 14C-Labeled Cycloartenol (specific isomer); 9β,19-Cyclo-5α-cholest-24-en-3β-ol, 4α,14-dimethyl-, (25-14C)-; Labeled Cycloartenol derivative; Isotopic Sterol Standard; Carbon-14 Labeled Triterpenoid Alcohol
EINECS	Contact for details

Quality Control

Every batch of 4α,14-Dimethyl-9β,19-Cyclo-(25-14C)5α-Cholest-24-En-3β-Ol is manufactured and tested under strict quality management systems. We guarantee high chemical and radiochemical purity, with specifications typically meeting or exceeding research-grade standards. Comprehensive analytical data, including HPLC, NMR, and MS analysis, is provided. A detailed Certificate of Analysis (COA) documenting identity, purity, specific activity, and radiochemical purity is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and ensure long-term stability of the labeled compound. Allow the vial to reach room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Chemical)
Radiochemical Purity	≥ 97%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Solubility	Soluble in chloroform, DMSO; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.