Description

Naphthol As-D Phosphate CAS NO 27580-13-8 is a specialized chromogenic substrate and biochemical reagent used as a phosphatase enzyme substrate in diagnostic and research applications. Its primary value lies in its ability to produce a highly colored, insoluble azo dye upon enzymatic hydrolysis, making it a critical component for precise histochemical and cytochemical staining. This product is essential for professionals in diagnostic kit manufacturing, life science research, and clinical pathology laboratories who require reliable and consistent staining reagents for enzyme activity detection.

Application

• Histochemical Staining: Primary substrate for the detection of alkaline phosphatase (ALP) and acid phosphatase (ACP) activity in tissue sections.
• Diagnostic Assays: Key component in ELISA and other immunoassay kits for signal generation and detection.
• Cell Biology Research: Used in cytochemical studies to localize and quantify phosphatase enzyme activity within cells.
• Microbiology: Employed in microbial identification systems that rely on phosphatase activity as a biochemical marker.
• Bone Disease Research: Applied in studies of osteoclast activity and bone metabolism due to its specificity for tartrate-resistant acid phosphatase (TRAP).
• In-situ Hybridization: Utilized as a chromogen in non-radioactive detection methods for nucleic acids.

Basic Information

Product Name	Naphthol As-D Phosphate
CAS No.	27580-13-8
Molecular Formula	C17H14NO6P
Molecular Weight	359.27 g/mol
Synonyms	Naphthol AS-D Phosphate; 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate; 3-Hydroxy-4'-methyl-2-naphthanilide phosphate; Naphthol AS-D phosphate ester; AS-D Naphthol phosphate; Phosphoric acid mono-(4-methyl-2-oxo-2H-naphtho[1,2-d][1,3]oxazol-7-yl) ester; Fast Red TR Salt substrate
EINECS	Contact for details

Quality Control

Our Naphthol As-D Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and residual solvent analysis, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to guarantee traceability, consistency, and compliance with your procedural requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines
Performance (Staining)	Yields intense, localized red precipitate with ALP/ACP

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.