Description

n-[3-[[(2,5-Dioxo-1-Pyrrolidinyl)Ethyl]Ethylamino]Phenyl]Acetamide CAS NO 27550-64-7 is a specialized chemical intermediate featuring a reactive N-hydroxysuccinimide (NHS) ester group and an aniline derivative structure. This bifunctional nature makes it a critical building block for creating stable amide bonds, primarily in bioconjugation and advanced material synthesis. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and polymer industries who require high-purity, reliable linkers for developing targeted therapies, biosensors, and functionalized surfaces.

Application

• Bioconjugation Reagent: Used to covalently link proteins, antibodies, or peptides to other molecules, nanoparticles, or solid supports for drug delivery systems and immunoassays.
• Diagnostic Kit Manufacturing: Serves as a key linker in the production of enzyme-linked immunosorbent assay (ELISA) kits, lateral flow assays, and other diagnostic tools.
• Polymer Functionalization: Employed to introduce reactive amine-handling sites onto polymer backbones, creating smart materials for biomedical and separation applications.
• Cross-linking Agent: Facilitates the cross-linking of biomolecules or synthetic polymers to form hydrogels or stable matrices for tissue engineering and chromatography.
• Pharmaceutical Intermediate: Acts as a precursor in the synthesis of more complex active pharmaceutical ingredients (APIs) and antibody-drug conjugates (ADCs).
• Surface Modification: Used to coat and functionalize surfaces (e.g., gold chips, magnetic beads) for biosensor development and lab-on-a-chip technologies.

Basic Information

Product Name	n-[3-[[(2,5-Dioxo-1-Pyrrolidinyl)Ethyl]Ethylamino]Phenyl]Acetamide
CAS No.	27550-64-7
Molecular Formula	C16H21N3O4
Molecular Weight	319.36 g/mol
Synonyms	N-[3-[[2-(2,5-Dioxo-1-pyrrolidinyl)ethyl]ethylamino]phenyl]acetamide; N-(3-((2-(2,5-Dioxopyrrolidin-1-yl)ethyl)(ethyl)amino)phenyl)acetamide; 3'-((2-(2,5-Dioxopyrrolidin-1-yl)ethyl)(ethyl)amino)acetanilide; NHS-PEG2-Aniline; Aniline-PEG2-NHS ester; Succinimidyl ester of aniline-PEG2-acetic acid; Acetamide, N-[3-[[2-(2,5-dioxo-1-pyrrolidinyl)ethyl]ethylamino]phenyl]-
EINECS	Contact for details

Quality Control

Our n-[3-[[(2,5-Dioxo-1-Pyrrolidinyl)Ethyl]Ethylamino]Phenyl]Acetamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification (NMR, IR), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider using desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.