Description

(1Alpha,2Alpha[N]-3H)-Cholesterol is a tritium-labeled derivative of cholesterol, a critical biomolecule and the precursor for steroid hormones. This labeled compound is essential for metabolic pathway tracing, receptor binding studies, and pharmacokinetic research where high-sensitivity detection is required. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers engaged in lipid metabolism and endocrinology studies.

Application

• Biochemical Research: As a radiolabeled tracer in studies of cholesterol absorption, biosynthesis, and metabolic flux.
• Drug Discovery & Development: For in vitro and in vivo assays to screen and evaluate compounds targeting cholesterol-related pathways (e.g., for hypercholesterolemia).
• Receptor Binding Assays: Used in competitive binding studies to characterize and quantify interactions with nuclear receptors and other cholesterol-binding proteins.
• Diagnostic Development: Serves as a key component in the development and calibration of radioimmunoassays (RIA) and other clinical diagnostic tests for lipid disorders.
• Metabolic Labeling: Enables the tracking and quantification of cholesterol and its derivatives in cell culture and animal models.
• Quality Control Reference: Acts as an internal standard in analytical methods such as LC-MS for the precise quantification of cholesterol in complex matrices.

Basic Information

Product Name	(1Alpha,2Alpha[N]-3H)-Cholesterol
CAS No.	27246-11-3
Molecular Formula	C27H45T3O
Molecular Weight	~ 389.7 g/mol (unlabeled base)
Synonyms	Cholesterol [1α,2α(n)-3H]; [1α,2α(n)-3H]Cholesterol; Tritiated Cholesterol; 3H-Cholesterol; (1α,2α-3H)Cholest-5-en-3β-ol; Radiolabeled Cholesterol; Cholesterol tritiate
EINECS	Contact for details

Quality Control

Every batch of (1Alpha,2Alpha[N]-3H)-Cholesterol is produced and analyzed under strict quality systems. We ensure high radiochemical purity and specific activity through validated analytical methods including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, purity, solvent composition, and analytical chromatograms. Our quality commitment supports research requiring reproducible and reliable tracer data.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in the original solvent as specified on the label. Due to its light-sensitive and highly volatile nature, minimize exposure to air and ambient temperature. For long-term storage, keep under inert atmosphere. Store in accordance with all local and national regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	10-60 Ci/mmol (Contact for specific value)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol, Toluene, or custom (as specified)
Concentration	1.0 mCi/mL (or as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.