Description

4-Ketocyclophosphamide is a key pharmaceutical intermediate and metabolite of the chemotherapeutic agent cyclophosphamide. This compound is of significant importance for research and development in oncology and pharmacology, serving as a critical reference standard and a precursor in synthetic pathways. It is primarily utilized by pharmaceutical manufacturers, contract research organizations (CROs), and academic institutions engaged in drug metabolism studies, impurity profiling, and the development of novel anticancer therapies.

Application

• Pharmaceutical Intermediate: Serves as a crucial building block in the synthesis of cyclophosphamide and related alkylating agents.
• Metabolite Reference Standard: Used as an analytical standard in bioanalytical methods (LC-MS, HPLC) for quantifying cyclophosphamide and its metabolites in pharmacokinetic and pharmacodynamic studies.
• Impurity Profiling: Employed as a certified reference material (CRM) to identify and control related substances in active pharmaceutical ingredient (API) batches according to ICH guidelines.
• Biomedical Research: Utilized in *in vitro* and *in vivo* studies to investigate the metabolic activation, mechanism of action, and toxicity profile of oxazaphosphorine drugs.
• Drug Discovery: Acts as a starting material or model compound for the design and synthesis of new anticancer prodrugs with improved efficacy and reduced side effects.

Basic Information

Product Name	4-Ketocyclophosphamide
CAS No.	27046-19-1
Molecular Formula	C7H15Cl2N2O3P
Molecular Weight	277.09 g/mol
Synonyms	4-Ketocyclophosphamide; Carboxyphosphamide; NSC 181815; 2-[Bis(2-chloroethyl)amino]-4-hydroperoxy-2-oxo-1,3,2-oxazaphosphorinane; 4-Hydroperoxycyclophosphamide; Cyclophosphamide Metabolite; 4-OOH-CPA
EINECS	Contact for details

Quality Control

Our 4-Ketocyclophosphamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR), purity determination by HPLC, and control of specific impurities. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, ensuring compliance with research and pharmaceutical grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5% Total impurities ≤ 2.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.