Description

Cefazolin CAS NO 25953-19-9 is a first-generation cephalosporin antibiotic belonging to the β-lactam class. It is valued for its potent bactericidal activity against a broad spectrum of Gram-positive and some Gram-negative bacteria. This product is essential for pharmaceutical manufacturers and research institutions developing sterile injectable formulations, active pharmaceutical ingredients (APIs), and reference standards.

Application

• Pharmaceutical API Manufacturing: Primary use as the active ingredient in sterile injectable antibiotic formulations.
• Research & Development: Serves as a critical reference standard in analytical method development, pharmacokinetic studies, and microbiological research.
• Hospital & Clinical Formulations: Used in the preparation of ready-to-use injectable solutions for the treatment of serious infections, including surgical prophylaxis.
• Veterinary Medicine: Employed in the development of antibiotic treatments for bacterial infections in animals.
• Quality Control Laboratories: Utilized as a certified reference material for assay, purity, and identification testing of commercial cefazolin products.

Basic Information

Product Name	Cefazolin
CAS No.	25953-19-9
Molecular Formula	C14H14N8O4S3
Molecular Weight	454.50 g/mol
Synonyms	Cefazolin Sodium; Cephazolin; Cefamezin; Ancef; Kefzol; Zolicef; 3-[[(5-Methyl-1,3,4-thiadiazol-2-yl)thio]methyl]-8-oxo-7-[2-(1H-tetrazol-1-yl)acetamido]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
EINECS	247-356-8

Quality Control

Our Cefazolin is manufactured and tested under strict quality management systems. We provide material that meets or exceeds relevant pharmacopeial standards (such as USP/EP) for identity, purity, and potency. A comprehensive Certificate of Analysis (COA) detailing all test results, including assay, related substances, residual solvents, and microbiological data, is provided with each batch to ensure full traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept under conditions that prevent exposure to excessive humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to standard
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	98.0% - 102.0% (on anhydrous basis)
pH (1% solution)	4.0 - 6.0
Water Content (KF)	≤ 6.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines
Bacterial Endotoxins	< 0.10 EU/mg
Sterility (where applicable)	Sterile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.