Description

4-(2-Iodoacetamido)-Tempo is a nitroxide spin label reagent featuring a reactive iodoacetamide functional group. This compound is critically important for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein structure, dynamics, and conformational changes. It is primarily needed by researchers and manufacturers in the fields of structural biology, biophysics, and pharmaceutical development for probing cysteine residues in proteins and peptides.

Application

• Site-Directed Spin Labeling (SDSL) for Electron Paramagnetic Resonance (EPR) spectroscopy studies.
• Probing protein structure and dynamics, including conformational changes and folding.
• Labeling of cysteine residues in peptides, proteins, and enzymes for biophysical analysis.
• Investigation of membrane protein topology and lipid-protein interactions.
• Use in distance measurements via pulsed EPR techniques like DEER (Double Electron-Electron Resonance).
• Research tool in structural biology and drug discovery programs.
• Functional studies of enzyme active sites and binding pockets.

Basic Information

Product Name	4-(2-Iodoacetamido)-Tempo
CAS No.	25713-24-0
Molecular Formula	C14H24IN3O2
Molecular Weight	393.26 g/mol
Synonyms	4-(2-Iodoacetamido)-2,2,6,6-tetramethylpiperidine-1-oxyl; 4-(Iodoacetamido)-TEMPO; 1-Oxyl-2,2,6,6-tetramethyl-4-piperidinyl iodoacetamide; Iodoacetamide-TEMPO; TEMPO-Iodoacetamide; 4-Iodoacetamido-TEMPO; 4-(Iodoacetamido)-2,2,6,6-tetramethylpiperidin-1-oxyl; SL-TEMPO-IAA
EINECS	Contact for details

Quality Control

Our 4-(2-Iodoacetamido)-Tempo is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed under an inert atmosphere when not in use to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
EPR Activity	Conforms
Melting Point	Contact for details
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.