Description

8-Bromoadenosine 5'-Triphosphate (CAS NO 23567-97-7) is a chemically modified nucleotide analog where a bromine atom is substituted at the 8-position of the adenine ring. This modification is critical for its role as a potent inhibitor of ATP-dependent enzymes and as a substrate analog in mechanistic biochemical studies. It is an essential research-grade biochemical for scientists in molecular biology, enzymology, and structural biology who require precise tools to probe nucleotide-binding sites and enzyme kinetics.

Application

• Enzyme Inhibition Studies: Used as a competitive inhibitor to investigate the active sites and catalytic mechanisms of ATP-dependent enzymes like kinases, ATPases, and polymerases.
• Biochemical Research: Serves as a substrate analog in enzymatic assays to study reaction pathways, binding affinities, and inhibition constants (Ki values).
• Structural Biology: Employed in X-ray crystallography and NMR spectroscopy to solve the three-dimensional structures of protein-nucleotide complexes.
• Nucleic Acid Research: Can be used to study the effects of modified nucleotides on nucleic acid structure, stability, and protein interactions.
• Drug Discovery & Development: Acts as a valuable tool compound for screening and validating new therapeutic targets that involve ATP-binding pockets.
• Metabolic Pathway Analysis: Applied in studies to dissect and understand complex metabolic networks where ATP is a key energy currency.

Basic Information

Product Name	8-Bromoadenosine 5'-Triphosphate
CAS No.	23567-97-7
Molecular Formula	C10H14BrN5O13P3
Molecular Weight	602.09 g/mol
Synonyms	8-Br-ATP; 8-Bromo-ATP; 8-Bromoadenosine-5'-triphosphate; Adenosine 5'-(tetrahydrogen triphosphate), 8-bromo-; 8-Bromo-adenosine-5'-triphosphate; 8-Bromoadenosine triphosphate; Br8ATP
EINECS	Contact for details

Quality Control

Our 8-Bromoadenosine 5'-Triphosphate is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated at -20°C. Allow the vial to warm to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.