Description

n-α-Benzoyl-L-Arginine p-Nitroanilide Hydrochloride is a chromogenic substrate specifically designed for the detection and quantification of trypsin-like proteolytic enzymes. This compound matters because it provides a reliable, colorimetric readout for enzymatic activity, which is critical for biochemical research, diagnostic assay development, and quality control in biopharmaceutical manufacturing. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries.

Application

• Primary substrate for the spectrophotometric assay of trypsin, thrombin, plasmin, and other serine proteases.
• Key reagent in enzymatic activity studies for kinetic analysis and inhibitor screening in drug discovery.
• Used in the development and quality control of diagnostic kits for pancreatic function and coagulation disorders.
• Essential component in biochemical research for studying protease mechanisms and signaling pathways.
• Applied in biopharmaceutical downstream processing to monitor and validate clearance of host cell proteases.
• Utilized in food science research for analyzing proteolytic activity in various products.

Basic Information

Product Name	n-α-Benzoyl-L-Arginine p-Nitroanilide Hydrochloride
CAS No.	21653-40-7
Molecular Formula	C20H25N7O4 • HCl
Molecular Weight	464.92 g/mol
Synonyms	BAPNA; Bz-Arg-pNA • HCl; Benzoyl-L-arginine 4-nitroanilide hydrochloride; Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; L-BAPA; BAPNA hydrochloride; Trypsin substrate BAPNA; N-Benzoyl-L-arginine-p-nitroanilide hydrochloride
EINECS	Contact for details

Quality Control

Our n-α-Benzoyl-L-Arginine p-Nitroanilide Hydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive enzymatic assays. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and reliable performance in your applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Absorbance (410 nm)	Specific range upon enzymatic cleavage
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.