Description

3-o-Methylfluorescein Phosphate Cyclohexylammonium Salt is a high-purity, chemically modified fluorescein derivative designed for advanced biochemical and diagnostic applications. This compound serves as a critical substrate for the detection and quantification of alkaline phosphatase (ALP) activity, offering superior sensitivity and stability in enzymatic assays. It is essential for researchers and manufacturers in the fields of molecular diagnostics, high-throughput screening (HTS), and immunoassay development, where precise enzymatic detection is paramount.

Application

• Substrate for Alkaline Phosphatase (ALP): Primary use in enzymatic assays for detecting ALP-conjugated antibodies in ELISA, Western blotting, and immunohistochemistry.
• Molecular Diagnostics: Key component in diagnostic kits for clinical biomarker detection and point-of-care testing devices.
• High-Throughput Screening (HTS): Utilized in pharmaceutical research for drug discovery campaigns targeting phosphatase activity.
• Cell-Based Assays: Enables real-time monitoring of intracellular phosphatase activity and signal transduction pathways.
• Biosensor Development: Integrated into sensor platforms for environmental monitoring or food safety testing.
• Research Reagent: Used in academic and industrial R&D for studying enzyme kinetics and inhibition.

Basic Information

Product Name	3-o-Methylfluorescein Phosphate Cyclohexylammonium Salt
CAS No.	21233-09-0
Molecular Formula	C27H28NO8P
Molecular Weight	525.49 g/mol
Synonyms	3-O-Methylfluorescein Phosphate Cyclohexylamine Salt; 3-OMFP Cyclohexylammonium Salt; Fluorescein 3-O-Methyl Phosphate, Cyclohexylammonium Salt; 3-OMFP; 3-O-Methylfluorescein Phosphate; 3-Methylfluorescein Phosphate Salt; ALP Substrate 3-OMFP; CAS 21233-09-0
EINECS	Contact for details

Quality Control

Our 3-o-Methylfluorescein Phosphate Cyclohexylammonium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and performance in standard enzymatic assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For extended storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Enzymatic Activity	Suitable for ALP detection
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.