Description

[γ-32P]-ATP is a radiolabeled form of adenosine triphosphate, where the gamma phosphate group contains the radioactive isotope phosphorus-32. This compound is a critical reagent in molecular biology and biochemistry research, enabling the sensitive detection and quantification of enzymatic activity. It is essential for researchers in pharmaceutical development, academic institutions, and diagnostic laboratories who require a high-specific-activity tracer for kinase assays, nucleic acid labeling, and metabolic studies.

Application

• Kinase Activity Assays: Used as the phosphate donor in assays to measure the activity of protein kinases, lipid kinases, and other ATP-dependent enzymes.
• Nucleic Acid Labeling: Employed in the in vitro labeling of DNA and RNA probes for techniques like Southern blotting, Northern blotting, and filter-binding assays.
• ATPase & Motor Protein Studies: Critical for investigating the hydrolysis cycle of ATPases, including ion pumps and cytoskeletal motor proteins like myosin and kinesin.
• Phosphorylation Mapping: Facilitates the identification and characterization of phosphorylation sites on proteins and other substrates.
• Metabolic Pathway Analysis: Acts as a tracer to study ATP utilization and turnover in cellular metabolic pathways.
• Drug Discovery Screening: Utilized in high-throughput screening (HTS) platforms to identify and characterize potential kinase inhibitors or activators.

Basic Information

Product Name	[γ-32P]-ATP
CAS No.	2964-07-0
Molecular Formula	C10H16N5O13P3 (with 32P in gamma position)
Molecular Weight	~507.18 g/mol (unlabeled ATP)
Synonyms	Adenosine 5'-[γ-32P]triphosphate; [γ-32P]ATP; ATP, [γ-32P]-labeled; Adenosine 5'-triphosphate, [γ-32P]-; 32P-ATP (γ-labeled); P-32 ATP; Radiochemical ATP; Triphosphoric acid, P'→5'-ester with adenosine, [γ-32P]-
EINECS	Contact for details

Quality Control

Every batch of [γ-32P]-ATP is manufactured and tested to meet stringent specifications for radiochemical purity and specific activity. Quality assurance includes verification by analytical techniques such as HPLC and TLC. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing the specific activity (Ci/mmol or Bq/mmol), radiochemical purity, concentration, and expiration date to ensure traceability and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in a designated radioactive materials freezer. The product is supplied in a suitable aqueous buffer solution (e.g., 10 mM Tris-HCl, 1 mM EDTA). Users must adhere to all local and national regulations for the safe handling, use, and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	>5000 Ci/mmol (Contact for current batch data)
Radiochemical Purity (HPLC)	≥ 95%
Concentration	~10 mCi/mL (Contact for current batch data)
Chemical Form	Tris salt, in aqueous buffer
Radionuclidic Purity	≥ 99% (32P)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.