Description

p-Nitrophenyl Butyrate is a high-purity organic ester compound widely used as a critical substrate in enzymatic assays and biochemical research. Its primary value lies in its role as a chromogenic substrate for detecting esterase and lipase activity, where its hydrolysis releases the yellow-colored p-nitrophenol, enabling quantitative spectrophotometric analysis. This product is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, where precise enzyme kinetics and activity measurements are required.

Application

• Enzymatic Assay Substrate: Primary substrate for spectrophotometric assays of esterases, lipases, and cholinesterases.
• Biochemical Research: Used in kinetic studies, enzyme inhibition testing, and mechanism-of-action research.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for liver function and other enzyme-level tests.
• Pharmaceutical QC: Employed in quality control testing of enzyme-based pharmaceuticals and in high-throughput screening (HTS) for drug discovery.
• Academic & Industrial R&D: Serves as a standard reagent in university laboratories and industrial R&D departments for method development and validation.
• Microbiology: Used in assays to study microbial esterase activity for environmental or industrial applications.

Basic Information

Product Name	p-Nitrophenyl Butyrate
CAS No.	2635-84-9
Molecular Formula	C10H11NO4
Molecular Weight	209.20 g/mol
Synonyms	4-Nitrophenyl butyrate; p-Nitrophenyl n-butyrate; Butyric acid 4-nitrophenyl ester; PNPB; 4-Nitrophenyl butanoate; PNP-Butyrate; 4-NPB; Butyryl 4-nitrophenyl ester
EINECS	220-132-4

Quality Control

Our p-Nitrophenyl Butyrate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by FT-IR and NMR, and verification of physical properties. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes. We support compliance with relevant research and quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed and stored in a dark location to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	70 - 74 °C
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.