Description

Naphthol As-Tr Phosphate CAS NO 2616-72-0 is a specialized biochemical reagent primarily used as a substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic activity measurement is required. It is essential for professionals in life sciences, clinical diagnostics, and pharmaceutical development who require reliable and high-purity substrates for enzyme-linked assays.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Serves as a key chromogenic substrate for detecting alkaline phosphatase conjugates.
• Clinical Diagnostics: Used in automated and manual diagnostic kits for detecting biomarkers in serum and other biological fluids.
• Immunohistochemistry (IHC): Employed as a substrate for visualizing antigen localization in tissue sections.
• Western Blotting: Facilitates the detection of proteins via alkaline phosphatase-conjugated antibodies.
• Microbiology Research: Utilized in assays to study bacterial or cellular phosphatase activity.
• Biochemical Research: Acts as a tool compound for studying enzyme kinetics and inhibition.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify enzyme modulators.

Basic Information

Product Name	Naphthol As-Tr Phosphate
CAS No.	2616-72-0
Molecular Formula	C23H16NO6P
Molecular Weight	433.35 g/mol
Synonyms	Naphthol AS-TR Phosphoric Acid Ester; 3-Hydroxy-4'-nitro-2-naphthanilide Phosphate; Naphthol AS-TR Phosphate; 4-Nitro-1-naphthol AS-TR Phosphate; AS-TR Phosphate; Alkaline Phosphatase Substrate; Naphthol AS-TR Acid Phosphate; 3-Hydroxy-N-(4-nitrophenyl)-2-naphthamide Phosphate
EINECS	220-049-5

Quality Control

Our Naphthol As-Tr Phosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by IR spectroscopy, to ensure it meets the high standards required for sensitive diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.