Description

Nonidet P40 Substitute CAS NO 2497-59-8 is a high-quality, non-ionic surfactant designed as a reliable alternative to the classic Nonidet P-40 (NP-40) detergent. This product is essential for applications requiring effective cell lysis and membrane protein solubilization while maintaining protein function and complex integrity. It is a critical reagent for research and development laboratories in the biotechnology, pharmaceutical, and molecular biology sectors, as well as for industrial biocatalysis and diagnostic kit manufacturing.

Application

• Cell Lysis and Protein Extraction: Gentle disruption of cell membranes for the isolation of cytoplasmic and nuclear proteins without denaturation.
• Membrane Protein Solubilization: Effective solubilization of integral membrane proteins for structural and functional studies.
• Immunoprecipitation (IP) and Co-IP: Used in lysis buffers to maintain protein-protein interactions for pull-down assays.
• Chromatin Immunoprecipitation (ChIP): Facilitates nuclear lysis for the study of protein-DNA interactions.
• Viral Research: Component of buffers for virus inactivation and the extraction of viral antigens or nucleic acids.
• Diagnostic Reagent Formulation: Used as a stabilizing agent and detergent in lateral flow assays and ELISA kits.
• Industrial Enzyme Preparation: Aids in the extraction and stabilization of enzymes from microbial or plant sources.

Basic Information

Product Name	Nonidet P40 Substitute
CAS No.	2497-59-8
Molecular Formula	C15H24O
Molecular Weight	220.35 g/mol
Synonyms	Octylphenol Ethoxylate; Octylphenoxypolyethoxyethanol; Polyethylene glycol tert-octylphenyl ether; Igepal CA-630; Triton X-100 (structurally similar); tert-Octylphenoxypoly(ethylenoxy)ethanol; (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol; Nonylphenol Ethoxylate Substitute
EINECS	219-697-6

Quality Control

Every batch of our Nonidet P40 Substitute is manufactured under a strict quality management system and undergoes rigorous analytical testing to ensure consistency, purity, and performance that meets the demanding requirements of life science research. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as ethylene oxide content, cloud point, and critical micelle concentration. Our quality commitment aligns with the standards expected for research-grade and in-vitro diagnostic (IVD) reagent applications.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a cool, dry, and well-ventilated area. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid
Identification (IR)	Conforms to structure
Assay (by EO content)	≥ 99.0%
Cloud Point (1% aq. sol.)	60 - 65 °C
pH (5% solution)	6.0 - 8.0
Moisture (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.