Description

2',7'-Dichlorofluorescin Diacetate is a vital fluorogenic substrate and chemical probe widely used in biochemical and cellular research. Its core value lies in its enzymatic conversion to the highly fluorescent 2',7'-dichlorofluorescein (DCF), making it an indispensable tool for detecting and quantifying reactive oxygen species (ROS). This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors. It serves as a critical reagent in assays for oxidative stress, cell viability, and antioxidant activity.

Application

• Oxidative Stress Detection: Primary substrate in the DCFH-DA assay for measuring intracellular levels of hydrogen peroxide and other reactive oxygen species (ROS) in live cells.
• Cell Viability & Cytotoxicity Assays: Used to assess cellular health, apoptosis, and the cytotoxic effects of pharmaceuticals or chemicals.
• Antioxidant Activity Screening: Employed in high-throughput screening (HTS) to evaluate the efficacy of potential antioxidant compounds in neutralizing free radicals.
• Neurological Research: Applied in studies of neurodegenerative diseases to monitor oxidative damage in neuronal cells.
• Environmental Toxicology: Utilized to study the oxidative impact of pollutants and toxins on various biological systems and model organisms.
• Pharmaceutical Development: Integral to in vitro testing during drug discovery for assessing compound-induced oxidative stress.
• Clinical Research: Supports research into diseases where oxidative stress is a contributing factor, such as cancer, diabetes, and cardiovascular conditions.

Basic Information

Product Name	2',7'-Dichlorofluorescin Diacetate
CAS No.	2044-85-1
Molecular Formula	C24H16Cl2O7
Molecular Weight	487.29 g/mol
Synonyms	DCFH-DA; 2',7'-Dichlorodihydrofluorescein Diacetate; Dichlorofluorescin Diacetate; H2DCFDA; DCFDA; 2',7'-Dichlorofluorescein Diacetate (common misnomer); 6-Carboxy-2',7'-dichlorofluorescin Diacetate; 2',7'-Dichloro-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one Diacetate
EINECS	Contact for details

Quality Control

Our 2',7'-Dichlorofluorescin Diacetate is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analysis, including HPLC purity assessment, spectroscopic identification, and verification of fluorogenic activity. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee the product meets the high standards required for pharmaceutical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under desiccated conditions. The product is light-sensitive (store away from light) and may degrade if exposed to moisture or elevated temperatures for prolonged periods. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Fluorogenic Activity	Conforms to reference standard
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.