Description

Biotin-PEG12-NHS Ester is a heterobifunctional crosslinking reagent designed for the stable, site-specific biotinylation of biomolecules. This compound is critical for researchers and developers requiring high-efficiency conjugation with minimal impact on the biological activity of target proteins, peptides, or other amine-containing substrates. It is an essential tool for scientists in pharmaceutical R&D, diagnostic assay development, and proteomics research, enabling advanced applications in drug delivery, biomarker detection, and affinity purification.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or imaging agents to monoclonal antibodies.
• Diagnostic Assay Reagent: Biotinylation of capture antibodies or antigens for use in ELISA, lateral flow assays, and other immunoassay platforms.
• Affinity Purification Probes: Creation of biotin-tagged ligands, peptides, or small molecules for streptavidin/avidin-based pull-down experiments.
• Cell Surface Labeling: Conjugation to amine groups on cell surface proteins for fluorescence-activated cell sorting (FACS) or microscopy studies.
• Protein and Peptide Modification: Introduction of a biotin tag onto recombinant proteins or synthetic peptides for immobilization or detection.
• Nanoparticle Functionalization: Surface modification of liposomes, polymeric nanoparticles, or quantum dots for targeted delivery systems.
• Microarray and Biosensor Fabrication: Immobilization of biomolecules on sensor surfaces via the biotin-streptavidin interaction.

Basic Information

Product Name	Biotin-PEG12-NHS Ester
CAS No.	1934296-88-4
Molecular Formula	C55H94N4O24S
Molecular Weight	1227.4 g/mol
Synonyms	Biotin-PEG12-NHS Ester; Biotinamidohexanoyl-PEG12-NHS Ester; Biotin-(PEG)12-NHS; Biotin-PEG12-NHS; NHS-PEG12-Biotin; N-Hydroxysuccinimide ester of Biotin-PEG12; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamido)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
EINECS	Contact for details

Quality Control

Our Biotin-PEG12-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	≥ 90% active ester by amine titration

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.