Description

Ac-Gly-Ala-Lys-Amc CAS NO 1926163-46-3 is a synthetic, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is a critical tool for researchers studying protease activity, particularly in the fields of biochemistry and drug discovery. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers for the quantitative measurement of specific enzyme kinetics and inhibition.

Application

• Protease Activity Assays: Used as a selective substrate for monitoring the catalytic activity of various serine and cysteine proteases in real-time.
• High-Throughput Screening (HTS): Enables rapid screening of potential drug candidates by measuring their inhibitory effects on target proteases in automated systems.
• Enzyme Kinetic Studies: Essential for determining kinetic parameters (Km, Vmax) and elucidating enzyme mechanisms in purified systems.
• Diagnostic Development: Serves as a key component in developing fluorometric diagnostic tests for diseases where specific protease levels are biomarkers.
• Biochemical Research: Employed in basic research to study protease function, regulation, and localization within cellular pathways.
• Quality Control in Biologics: Used to assess the presence and activity of residual proteases in biopharmaceutical manufacturing processes.

Basic Information

Product Name	Ac-Gly-Ala-Lys-Amc
CAS No.	1926163-46-3
Molecular Formula	C24H34N6O7
Molecular Weight	518.57 g/mol
Synonyms	Acetyl-Gly-Ala-Lys-7-Amino-4-methylcoumarin; Ac-Gly-Ala-Lys-AMC; Ac-GAK-AMC; N-Acetyl-Glycyl-L-alanyl-L-lysyl-7-amino-4-methylcoumarin; Fluorogenic Peptide Substrate Ac-GAK-AMC; Protease Substrate IV (related); L-Lysine, N2-acetyl-N6-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl)carbonyl]-L-alanylglycyl-; AMC-labeled peptide substrate
EINECS	Contact for details

Quality Control

Our Ac-Gly-Ala-Lys-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.