Description

Fitc-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh (Contains Fitc Isomer I) is a specialized, high-purity fluorescent peptide substrate designed for sensitive biochemical research. This compound is critical for studying caspase-3 and related apoptotic pathways, offering researchers a reliable tool for enzyme activity assays. It is primarily utilized by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers who require precise and reproducible results in protease detection and cell biology studies.

Application

• Caspase-3 Activity Assays: A key fluorescent substrate for the detection and quantification of caspase-3 enzyme activity in apoptosis research.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential inhibitors or activators of caspase enzymes.
• Biochemical Research: Serves as a fundamental reagent for studying protease kinetics, specificity, and mechanism of action in controlled in vitro systems.
• Cell-Based Assays: Employed in live-cell imaging and flow cytometry to monitor apoptotic events in real-time within cellular models.
• Diagnostic Development: Acts as a core component in the development of research-use-only (RUO) diagnostic kits for measuring apoptotic markers.
• Quality Control in Biologics: Used in the biopharmaceutical industry to assess the presence of host cell proteases during downstream processing.

Basic Information

Product Name	Fitc-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh (Contains Fitc Isomer I)
CAS No.	1926163-32-7
Molecular Formula	C66H81N13O22
Molecular Weight	1400.42 g/mol
Synonyms	FITC-DEVD-AFC (alternative naming convention); Fluorescein Isothiocyanate-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH; Caspase-3 Substrate IV (FITC-labeled); Ac-DEVD-AFC (common core sequence reference); Fluorogenic Caspase-3 Substrate; FITC-YVADAPK(Dnp)-OH; Apoptosis Detection Substrate; Ac-Asp-Glu-Val-Asp-AFC (related commercial name)
EINECS	Contact for details

Quality Control

Every batch of Fitc-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with the high-purity standards required for life science research. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (typically >95% by HPLC), and performance in functional assays.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Functional Activity	Conforms to reference in caspase-3 assay
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.