Description

H-Glu(Edans)-Pro-Leu-Phe-Ala-Glu-Arg-Lys(Dabcyl)-Oh is a high-purity, synthetic fluorogenic peptide substrate designed for sensitive protease activity assays. Its core value lies in the FRET (Fluorescence Resonance Energy Transfer) pair formed by the Edans donor and Dabcyl acceptor, which provides a robust signal-to-noise ratio upon cleavage. This compound is essential for researchers and quality control laboratories in the pharmaceutical and biotechnology sectors requiring precise enzymatic measurements.

Application

• Protease Activity Assays: Specifically designed as a substrate for monitoring the activity of various proteolytic enzymes, including matrix metalloproteinases (MMPs) and other serine or cysteine proteases.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors as therapeutic candidates.
• Biochemical Research: A critical tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial research settings.
• Diagnostic Kit Development: Serves as a key component in the development of fluorometric diagnostic tests and kits for disease biomarkers related to protease activity.
• Quality Control in Biologics: Employed to assess the presence and activity of host cell proteases during the manufacturing and purification processes of biologic drugs.

Basic Information

Product Name	H-Glu(Edans)-Pro-Leu-Phe-Ala-Glu-Arg-Lys(Dabcyl)-Oh
CAS No.	1914987-47-5
Molecular Formula	C71H98N18O22S2
Molecular Weight	1547.78 g/mol
Synonyms	Edans-Glu-Pro-Leu-Phe-Ala-Glu-Arg-Lys(Dabcyl); FRET Peptide Substrate MMP; Fluorogenic Peptide Substrate; Dabcyl-Glu-Pro-Leu-Phe-Ala-Glu-Arg-Lys(Edans)-OH; Mca-PLGL-Dpa-AR-NH2 (related analog); MMP Substrate, Fluorogenic; 5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid (Edans) and 4-((4-(Dimethylamino)phenyl)azo)benzoic acid (Dabcyl) labeled peptide; (EDANS)Glu-Pro-Leu-Phe-Ala-Glu-Arg-Lys(DABCYL)-OH
EINECS	Contact for details

Quality Control

Our H-Glu(Edans)-Pro-Leu-Phe-Ala-Glu-Arg-Lys(Dabcyl)-Oh is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, peptide content, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Peptide Content	≥ 70% (by weight)
Absorption (λ max)	Characteristic of Edans/Dabcyl FRET pair
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.