Description

Tamra-Peg4-Dbco is a fluorescent dye-conjugated linker reagent designed for bioorthogonal click chemistry applications. This compound is engineered to enable the sensitive labeling, detection, and tracking of biomolecules in complex biological systems. It is an essential tool for researchers and developers in the fields of bioconjugation, proteomics, and advanced diagnostics, where precise and stable fluorescent tagging is required.

Application

• Bioconjugation and Probe Development: Used for site-specific labeling of antibodies, proteins, peptides, and oligonucleotides with a bright, photostable TAMRA fluorophore.
• Live-Cell Imaging and Tracking: Facilitates real-time visualization and tracking of cellular processes via strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry with minimal cellular toxicity.
• Diagnostic Assay Development: Serves as a key component in developing fluorescent immunoassays, lateral flow tests, and in-situ hybridization (FISH) probes for high-sensitivity detection.
• Materials Science and Surface Functionalization: Enables the creation of fluorescently tagged hydrogels, nanoparticles, and polymer scaffolds for biomedical engineering and sensor applications.
• Chemical Biology and Proteomics: Employed in activity-based protein profiling (ABPP) and target identification studies to label and isolate specific enzyme families.
• Pre-clinical Research: Used in pharmacokinetic and biodistribution studies to fluorescently tag small molecules or nanoparticles for in vivo imaging.

Basic Information

Product Name	Tamra-Peg4-Dbco
CAS No.	1895849-41-8
Molecular Formula	C58H71N5O13
Molecular Weight	1046.23 g/mol
Synonyms	5-TAMRA-PEG4-DBCO; TAMRA-PEG4-Dibenzocyclooctyne; 6-Carboxytetramethylrhodamine-PEG4-DBCO; DBCO-PEG4-5-TAMRA; 5(6)-TAMRA SE-PEG4-DBCO; Tetramethylrhodamine-PEG4-DBCO Conjugate; N-(6-(3-(3-(2-(2-(3-(2,5-Dioxopyrrolidin-1-yl)oxy)-2-oxoethoxy)ethoxy)ethoxy)propyl)-1,4,7,10-tetraoxa-13-azacyclopentadecan-13-yl)-6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanamido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid (Isomer mixture)
EINECS	Contact for details

Quality Control

Every batch of Tamra-Peg4-Dbco is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR, and mass spectrometry. Our quality commitment aligns with the standards required for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material should be handled under inert atmosphere when appropriate for prolonged experimental use.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~90,000 cm-1M-1 at 555 nm
Fluorescence Emission	~580 nm
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.