Description

6-Chloro-4-Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of enzyme activity. This compound matters for its high specificity and sensitivity in biochemical assays, enabling precise research and diagnostic applications. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit production sectors.

Application

• Enzymatic Substrate for N-Acetyl-β-D-glucosaminidase (NAGase): A key fluorogenic substrate for detecting and quantifying NAGase activity, an important biomarker for renal function and certain lysosomal storage disorders.
• Biochemical Research and Assay Development: Used in high-throughput screening (HTS) and kinetic studies to investigate enzyme kinetics, inhibition, and mechanism of action.
• Diagnostic Reagent Manufacturing: Incorporated into clinical diagnostic kits for the automated or manual measurement of enzyme levels in biological samples like serum or urine.
• Pharmaceutical Quality Control: Employed in potency assays for enzyme-based therapeutics and in stability testing of biopharmaceutical products.
• Microbiology and Cell Biology: Useful in assays to study bacterial or fungal enzyme activity and for probing glycosidase functions within cellular pathways.

Basic Information

Product Name	6-Chloro-4-Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Glucopyranoside
CAS No.	1867162-08-0
Molecular Formula	C₁₈H₂₀ClNO₉
Molecular Weight	429.80 g/mol
Synonyms	6-Chloro-4-methylumbelliferyl N-acetyl-β-D-glucosaminide; 4-MU-β-GlcNAc (6-Chloro derivative); 6-Chloro-4-MU-β-GlcNAc; NAGase Fluorogenic Substrate (6-Chloro-4-MU); 2-Acetamido-2-deoxy-β-D-glucopyranoside, 6-chloro-4-methylumbelliferyl ester; β-N-Acetylglucosaminidase Substrate; 6-Cl-4-MU-GlcNAc
EINECS	Contact for details

Quality Control

Our 6-Chloro-4-Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.