Description

Biotin-Peg3-Azide is a high-purity, heterobifunctional crosslinking reagent designed for bioconjugation and click chemistry applications. Its molecular architecture combines the high-affinity biotin-streptavidin binding system with a polyethylene glycol (PEG) spacer and a terminal azide group, enabling efficient, site-specific labeling of biomolecules. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, diagnostics, and materials science who require reliable and versatile tools for constructing complex molecular assemblies.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides via strain-promoted azide-alkyne cycloaddition (SPAAC) or copper-catalyzed click chemistry (CuAAC).
• Diagnostic Assay Development: Conjugation of detection molecules (e.g., fluorophores, enzymes) to targeting ligands for ELISA, lateral flow assays, and other diagnostic platforms.
• Drug Delivery System Fabrication: Functionalization of nanoparticles, liposomes, and polymer carriers to attach targeting moieties for enhanced therapeutic delivery.
• Affinity Purification and Pull-Down Assays: Immobilization of biotinylated molecules onto streptavidin/avidin resins for the isolation and study of protein complexes or biomarkers.
• Surface Engineering and Biointerface Creation: Modification of biosensor surfaces, microarrays, and cell culture substrates to create bioactive interfaces.
• Chemical Biology and Probe Synthesis: Construction of activity-based probes and chemical reporters for studying biological processes in live cells.

Basic Information

Product Name	Biotin-Peg3-Azide
CAS No.	1859110-99-8
Molecular Formula	C₂₀H₃₆N₆O₆S
Molecular Weight	488.61 g/mol
Synonyms	Biotin-PEG3-N3; Biotin-PEG3-azide; Biotin-PEG-triazole-azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-azidoethoxy)ethoxy)ethyl)pentanamide; N-(Biotinoyl)-N'-(3-azidopropyl)-3,6-dioxaoctanediamine; Azido-PEG3-biotin; Biotin-azide-PEG3; Biotinylated PEG azide
EINECS	Contact for details

Quality Control

Our Biotin-Peg3-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support development under cGMP guidelines and can supply material suitable for research and non-GMP commercial applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.