Description

Methyltetrazine-Sulfo-NHS Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound matters for its unique combination of a bioorthogonal methyltetrazine group and an amine-reactive NHS ester, enabling rapid, catalyst-free click chemistry for labeling biomolecules. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and in vitro diagnostics who require efficient and stable linkage of probes, drugs, or surfaces to proteins, antibodies, and other amine-containing targets.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the tetrazine-trans-cyclooctene (TCO) inverse electron demand Diels-Alder (IEDDA) reaction.
• Protein and Peptide Labeling: Covalent attachment of fluorescent dyes, biotin, or other functional tags for imaging, detection, and pull-down assays.
• Surface Functionalization: Modification of biosensor chips, microarray slides, or nanoparticle surfaces with tetrazine groups for subsequent capture or detection applications.
• Live-Cell Imaging Probes: Construction of turn-on or targeted imaging agents for real-time visualization of biological processes.
• Diagnostic Reagent Synthesis: Key building block for creating immunoassay components and other diagnostic tools requiring stable, high-affinity linkages.
• Polymer and Biomaterial Science: Incorporation into hydrogels or polymeric scaffolds for controlled drug release or cell culture applications.

Basic Information

Product Name	Methyltetrazine-Sulfo-NHS Ester
CAS No.	1821017-46-2
Molecular Formula	C14H13N5O7S
Molecular Weight	395.35 g/mol
Synonyms	6-Methyl-1,2,4,5-tetrazine-3-carboxylic acid, 2,5-dioxopyrrolidin-1-yl ester, sulfonated; Sulfo-NHS-Methyltetrazine; Methyltetrazine-PEG4-Sulfo-NHS Ester; 3-(6-Methyl-1,2,4,5-tetrazin-3-yl)-N-(2,5-dioxopyrrolidin-1-yl)propanamide, sulfonated derivative; Methyl-Tz-Sulfo-NHS; m-Tetrazine-Sulfo-NHS Ester; Bioorthogonal Crosslinker, Methyltetrazine NHS Ester
EINECS	Contact for details

Quality Control

Every batch of Methyltetrazine-Sulfo-NHS Ester is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and consistent performance. Our quality system is designed to meet the stringent requirements of research and pre-clinical development. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing purity (by HPLC), identity confirmation (by NMR and MS), and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. Store away from strong bases and organic solvents.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.