Description

Spdp-Peg6-Nhs Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group, connected by a flexible, hydrophilic hexaethylene glycol (PEG6) spacer. This compound is engineered to facilitate the stable conjugation of biomolecules, such as antibodies or proteins, to other entities like surfaces, particles, or drugs, while minimizing aggregation and preserving biological activity. It is an essential tool for researchers and developers in the fields of bioconjugation, diagnostic assay development, antibody-drug conjugate (ADC) research, and targeted drug delivery systems.

Application

• Antibody-Drug Conjugate (ADC) Linker: For constructing stable, cleavable linkages between cytotoxic payloads and monoclonal antibodies.
• Protein/Peptide Labeling: Conjugation of fluorescent dyes, enzymes, or other reporter molecules to proteins via amine and thiol groups.
• Surface Functionalization: Immobilization of proteins or peptides onto biosensor chips, microarray slides, or nanoparticles for diagnostic and research applications.
• PEGylation Reagent: Introducing a hydrophilic PEG spacer to biomolecules to enhance solubility, reduce immunogenicity, and improve pharmacokinetic profiles.
• Biomaterial Crosslinking: Creating crosslinked hydrogels or modifying polymer scaffolds for tissue engineering and controlled release.
• Diagnostic Reagent Synthesis: Key component in the development of enzyme-linked immunosorbent assays (ELISAs) and other immunoassays.

Basic Information

Product Name	Spdp-Peg6-Nhs Ester
CAS No.	1818294-32-4
Molecular Formula	C₃₃H₅₀N₄O₁₃S₂
Molecular Weight	778.9 g/mol
Synonyms	SPDP-PEG6-NHS Ester; NHS-PEG6-SPDP; N-Succinimidyl 3-((2-(2-(2-(2-(2-(3-(2-pyridyldithio)propanamido)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)carbamoyl)propanoate; Succinimidyl PEG6 SPDP; PEG6-SPDP Crosslinker; Heterobifunctional PEG Crosslinker, NHS-PEG6-PDP; 3-(2-Pyridyldithio)-N-{2-[2-(2-{2-[2-(2-{3-[(2,5-dioxopyrrolidin-1-yl)oxy]-3-oxopropyl}amino)ethoxy]ethoxy}ethoxy)ethoxy]ethyl}propanamide
EINECS	Contact for details

Quality Control

Our Spdp-Peg6-Nhs Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency for critical research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements for GMP-grade intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	≥ 90% (by Ellman's assay or equivalent)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.