Description

Biotinyl-Hepcidin-25 (Human) CAS NO 1815618-07-5 is a biotinylated, bioactive peptide analog of the key iron-regulatory hormone hepcidin. This modification is critical for research and diagnostic applications, enabling highly sensitive detection, purification, and quantification of hepcidin and its binding partners. It is an essential reagent for pharmaceutical R&D, clinical research, and diagnostic kit development focused on iron metabolism disorders, anemia of chronic disease, and related therapeutic pathways.

Application

• Biomarker Research & Assay Development: Used as a labeled standard or capture reagent in ELISA, Western blot, and other immunoassays to study hepcidin levels and function.
• Drug Discovery & Screening: Serves as a key tool for high-throughput screening (HTS) to identify and characterize novel compounds that modulate the hepcidin-ferroportin interaction.
• Diagnostic Kit Manufacturing: Integrated into commercial in-vitro diagnostic (IVD) test kits for the precise measurement of hepcidin in human serum or plasma.
• Pull-down & Affinity Purification: Utilizes the biotin tag for streptavidin-based isolation of hepcidin-binding proteins or receptors from complex biological samples.
• Mechanistic Studies: Enables detailed investigation of hepcidin's role in iron homeostasis, inflammation, and associated metabolic pathways.
• Quality Control Reference Standard: Acts as a certified reference material (CRM) for the calibration and validation of analytical methods in regulated environments.

Basic Information

Product Name	Biotinyl-Hepcidin-25 (Human)
CAS No.	1815618-07-5
Molecular Formula	C150H246N46O44S9
Molecular Weight	3484.3 g/mol (theoretical)
Synonyms	Biotin-Hepcidin-25; Biotinylated Hepcidin (1-25), Human; Hepcidin-25 (Human), Biotin conjugate; HAMP (1-25), Biotinylated; LEAP-1 (1-25), Biotinylated; Liver-expressed antimicrobial peptide 1, Biotinylated; Iron-regulatory hormone peptide, Biotin conjugate; Biotin-DTHFPICIFCCGCCHRSKCGMCCKT
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Biotinyl-Hepcidin-25 (Human) is characterized using advanced analytical techniques including HPLC, MS, and amino acid analysis to confirm identity, purity, and correct biotinylation. Certificates of Analysis (COA) detailing purity, peptide content, and analytical data are available upon request. We adhere to quality management principles suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider aliquoting to minimize freeze-thaw cycles and maintain stability. Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Molecular weight confirmed by mass spectrometry
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by amino acid analysis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.