Description

Biotin-Peg6-Mal CAS NO 1808990-66-0 is a heterobifunctional crosslinker featuring a biotin group for affinity capture and a maleimide (Mal) group for thiol-specific conjugation, connected by a flexible hexaethylene glycol (PEG6) spacer. This compound is critical for creating stable, site-specific bioconjugates with enhanced solubility and reduced immunogenicity. It is an essential reagent for researchers and developers in biopharmaceuticals, diagnostic assay development, and targeted drug delivery systems who require precise and efficient labeling of proteins, antibodies, and peptides.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via engineered cysteine residues.
• Diagnostic Probe Labeling: Functionalization of proteins, peptides, or nanoparticles for use in ELISA, lateral flow assays, and other biosensors.
• Affinity Chromatography Ligand Immobilization: Covalent attachment of thiol-containing biomolecules to solid supports for purification or capture studies.
• Surface Plasmon Resonance (SPR) & Biacore Studies: Immobilization of thiolated ligands on sensor chips for real-time interaction analysis.
• Fluorescent & Imaging Agent Conjugation: Preparation of biotinylated, target-specific imaging probes for cellular and in vivo studies.
• Protein & Peptide Engineering: Introduction of biotin handles into protein structures for pull-down assays, Western blot detection, or avidin/streptavidin-based applications.
• Nanoparticle Functionalization: Modification of gold nanoparticles or liposomes for targeted delivery and diagnostic applications.
• Stability & Solubility Enhancement: The PEG spacer improves the pharmacokinetic properties of bioconjugates by increasing aqueous solubility and reducing aggregation.

Basic Information

Product Name	Biotin-Peg6-Mal
CAS No.	1808990-66-0
Molecular Formula	C36H60N4O13S
Molecular Weight	788.95 g/mol
Synonyms	Biotin-PEG6-maleimide; Maleimide-PEG6-Biotin; Biotin-(PEG)6-maleimide; Biotin-PEG6-Mal; Biotin hexaethylene glycol maleimide; 5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[2-[2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]pentanamide
EINECS	Contact for details

Quality Control

Our Biotin-Peg6-Mal is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and functional integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research and development documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Integrity	Maleimide & Biotin activity confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.