Description

Dde Biotin-Peg4-Alkyne is a multifunctional chemical reagent designed for advanced bioconjugation and click chemistry applications. Its core value lies in the strategic combination of a cleavable Dde protecting group, a biotin affinity tag, a hydrophilic PEG spacer, and a terminal alkyne handle, enabling precise, multi-step labeling and purification workflows. This compound is essential for researchers and manufacturers in proteomics, drug discovery, and diagnostic assay development who require high-purity, reliable linkers for site-specific modification of biomolecules.

Application

• Proteomic Research: Site-specific labeling of proteins and peptides for pull-down assays, mass spectrometry analysis, and protein interaction studies.
• Antibody-Drug Conjugate (ADC) Development: Facilitating the construction of cleavable linker-payload systems for targeted cancer therapies.
• Diagnostic Probe Synthesis: Building blocks for fluorescent or enzymatic diagnostic probes where controlled release of a signal is required.
• Biomaterial Functionalization: Surface modification of nanoparticles, hydrogels, and chips with bioactive molecules for biosensing and tissue engineering.
• Click Chemistry Toolkits: A key reagent in CuAAC (Copper-Catalyzed Azide-Alkyne Cycloaddition) or strain-promoted click chemistry kits for bioconjugation.
• Affinity Purification: Temporary immobilization of biomolecules on streptavidin resins, with subsequent elution via Dde deprotection.

Basic Information

Product Name	Dde Biotin-Peg4-Alkyne
CAS No.	1802908-00-4
Molecular Formula	C₄₀H₆₄N₆O₁₂S
Molecular Weight	853.04 g/mol
Synonyms	1-(4,4-Dimethyl-2,6-dioxocyclohexylidene)-3-[2-[2-[2-[2-[5-(4,5-dihydro-1H-imidazol-2-yl)pentanoylamino]-5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethyl 5-hexynamide; Biotin-PEG4-Dde-Alkyne; Dde-Biotin-PEG4-Alkyne; N-(Dde)-Biotin-PEG4-Propargyl; Alkyne-PEG4-Biotin-Dde; Biotin-Dde-PEG4-Alkyne; Propargyl-PEG4-Dde-Biotin
EINECS	Contact for details

Quality Control

Our Dde Biotin-Peg4-Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all critical quality attributes. Our production adheres to cGMP principles where applicable, ensuring reliability for research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.