Description

Methyltetrazine-Peg4-Nhs Ester CAS NO 1802907-92-1 is a heterobifunctional crosslinking reagent designed for bioconjugation and labeling applications. Its molecular architecture combines a bioorthogonal methyltetrazine group for rapid, catalyst-free click chemistry with an amine-reactive NHS ester, bridged by a hydrophilic, flexible tetraethylene glycol (PEG4) spacer. This product is essential for researchers and developers requiring efficient, site-specific modification of proteins, antibodies, peptides, and other biomolecules under mild, aqueous conditions. It is primarily utilized in the pharmaceutical, diagnostics, and life science research sectors for creating advanced antibody-drug conjugates (ADCs), imaging probes, and functionalized surfaces.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the tetrazine-trans-cyclooctene (TCO) inverse electron demand Diels-Alder (IEDDA) reaction.
• Fluorescent & Imaging Probe Synthesis: Labeling of proteins, peptides, and nanoparticles with fluorophores, MRI contrast agents, or radionuclide chelators for in vitro and in vivo diagnostics.
• Surface Functionalization: Immobilization of biomolecules onto chips, beads, or biosensor surfaces for microarray, SPR, or diagnostic assay development.
• Protein & Peptide Modification: Introduction of a bioorthogonal handle onto lysine residues for subsequent two-step labeling strategies, enabling multiplexing and minimal perturbation.
• Polymer & Biomaterial Science: Synthesis of functionalized hydrogels, smart polymers, and drug delivery systems with clickable tetrazine moieties.
• Chemical Biology Tool Development: Creation of activity-based probes and affinity tags for target identification and proteomics studies.

Basic Information

Product Name	Methyltetrazine-Peg4-Nhs Ester
CAS No.	1802907-92-1
Molecular Formula	C28H40N6O9
Molecular Weight	604.66 g/mol
Synonyms	5-Methyl-1,2,4,5-tetrazin-3(2H)-one-PEG4-NHS ester; Methyltetrazine-PEG4-NHS; 3-(5-Methyl-1,2,4,5-tetrazin-3(2H)-on-6-yl)propanoic acid-PEG4-NHS ester; m-Tetrazine-PEG4-NHS ester; 6-(3-(2,5-Dioxopyrrolidin-1-yl)oxy)-3,6,9,12-tetraoxatetradecyl)-5-methyl-1,2,4,5-tetrazin-3(2H)-one; Methyl-Tz-PEG4-NHS; Bioorthogonal linker NHS-PEG4-methyltetrazine; Tetrazine-PEG4-NHS Ester (methyl substituted)
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing batch-specific results to ensure traceability and compliance with your research and development protocols. For cGMP-grade material or custom specifications, please contact our technical sales team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Orange to red solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.