Description

5-Tamra-Amyloid β-Protein (1-42) is a fluorescently labeled derivative of the amyloid-beta peptide, a key biomarker in Alzheimer's disease research. This high-purity, synthetic peptide is conjugated with the 5-TAMRA fluorophore, enabling sensitive detection and visualization in advanced biochemical assays. It is an essential tool for researchers and diagnostic developers in the fields of neuroscience, pharmaceutical discovery, and clinical diagnostics, facilitating studies on protein aggregation, biomarker detection, and therapeutic screening.

Application

• Biomarker Research & Development: Critical for in vitro and ex vivo studies of amyloid-beta aggregation kinetics and neurotoxicity in Alzheimer's disease models.
• Diagnostic Assay Development: Used as a fluorescent tracer or standard in the development of ELISA, Western blot, and other immunoassay platforms for detecting Aβ(1-42).
• High-Content Screening (HCS): Enables automated, cell-based imaging assays in pharmaceutical discovery to identify compounds that modulate amyloid-beta pathology.
• Fluorescence Microscopy & Imaging: Serves as a probe for visualizing amyloid plaques and oligomers in tissue sections and cellular models.
• Binding & Interaction Studies: Utilized in surface plasmon resonance (SPR), fluorescence polarization (FP), and other biophysical techniques to study protein-protein interactions.
• Quality Control Reference Standard: Acts as a certified reference material (CRM) for calibrating and validating analytical methods in diagnostic manufacturing.

Basic Information

Product Name	5-Tamra-Amyloid β-Protein (1-42)
CAS No.	1802087-80-4
Molecular Formula	C227H340N56O68S
Molecular Weight	~4870.5 g/mol (monoisotopic)
Synonyms	5-TAMRA-Aβ(1-42); 5-Carboxytetramethylrhodamine-labeled Amyloid Beta Peptide (1-42); TAMRA-Amyloid β-Protein (1-42); Fluorescent Aβ42; Aβ(1-42)-TAMRA conjugate; 5-TAMRA-Aβ42; Amyloid-β (1-42) labeled with 5-TAMRA; TAMRA-Aβ(1-42) human
EINECS	Contact for details

Quality Control

Every batch of 5-Tamra-Amyloid β-Protein (1-42) is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Purity is verified by reverse-phase HPLC (≥95%) and identity confirmed by mass spectrometry (MS). Our quality commitment ensures the product meets the stringent requirements for research and in vitro diagnostic (IVD) use. A detailed Certificate of Analysis (COA) providing batch-specific data on purity, content, and fluorescence properties is supplied with each order.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use vials to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Reddish lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by amino acid analysis)
Fluorophore-to-Peptide Ratio	~1:1 (by absorbance)
Solubility	Soluble in DMSO, DMF, or basic aqueous buffers
Endotoxin Level	< 1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.