Description

Mca-Amyloid β/A4 Protein Precursor770(667-676)-Lys(Dnp)-Arg-Arg Amide Trifluoroacetate Salt is a specialized, sequence-specific fluorogenic peptide substrate designed for high-sensitivity protease activity assays. This compound is critical for researchers investigating the enzymatic processing of the amyloid precursor protein (APP), a key pathway in Alzheimer's disease research and neurodegenerative disorder studies. It is primarily utilized by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic developers focused on protease inhibitor screening and mechanistic enzymology.

Application

• Protease Activity Assays: Specifically designed as a fluorogenic substrate for β-secretase (BACE-1) and related aspartyl proteases involved in APP cleavage.
• Alzheimer's Disease Research: Enables the study of amyloid-beta peptide generation pathways and the evaluation of therapeutic compounds targeting secretase enzymes.
• High-Throughput Screening (HTS): Used in automated platforms for the discovery and optimization of novel protease inhibitors for neurological disorders.
• Biochemical Mechanism Studies: Facilitates kinetic analysis and determination of enzymatic parameters (Km, Vmax, Ki) for proteases cleaving the APP sequence.
• Diagnostic Development: Serves as a key reagent in developing in-vitro diagnostic tests for measuring secretase activity in biological samples.
• Cell-Based Assays: Applicable in cellular models to monitor APP processing and the effects of genetic or pharmacological interventions in real-time.

Basic Information

Item	Detail
Product Name	Mca-Amyloid β/A4 Protein Precursor770(667-676)-Lys(Dnp)-Arg-Arg Amide Trifluoroacetate Salt
CAS No.	1802078-33-6
Molecular Formula	C71H102N24O19 • C2HF3O2
Molecular Weight	Contact for details
Synonyms	Mca-APP(667-676)-Lys(Dnp)-Arg-Arg-NH2 TFA Salt; Fluorogenic BACE-1 Substrate; (7-Methoxycoumarin-4-yl)acetyl-SEVNL-DAEFR-Lys(Dnp)-Arg-Arg-NH2 TFA; SEVNL-DAEFR-K(Dnp)-RR-amide TFA Salt; APP770 Fluorogenic Peptide Substrate; β-Secretase Substrate I; BACE-1 Fluorogenic Substrate; Mca-SEVNLDAEFRK(Dnp)RR-NH2
EINECS	Contact for details

Quality Control

Every batch of our Mca-Amyloid β/A4 Protein Precursor770(667-676)-Lys(Dnp)-Arg-Arg Amide Trifluoroacetate Salt is manufactured and analyzed under strict quality management systems. We guarantee high purity and batch-to-batch consistency essential for reproducible research results. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), peptide content, and identity confirmation is provided with each shipment. Our quality protocols are designed to meet the rigorous standards of pharmaceutical R&D and analytical biochemistry.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. Aliquot into single-use vials after reconstitution to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Peptide Content	≥ 70.0% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.