Description

Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770(667-675)-Lys(Dnp) Amide Ammonium Salt is a specialized, high-purity synthetic peptide sequence designed for advanced biochemical research. This compound is a critical tool for studying the proteolytic processing and pathological aggregation of the amyloid precursor protein, which is central to Alzheimer's disease research. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic developers focused on neurodegenerative diseases and protease activity assays.

Application

• Protease Substrate & Enzyme Kinetics: Used as a specific, fluorogenic substrate for studying the activity and inhibition of β-secretase 1 (BACE1) and related aspartyl proteases.
• Alzheimer's Disease Research: Enables the investigation of amyloid-beta peptide generation pathways, aiding in the development of therapeutic targets and diagnostic markers.
• High-Throughput Screening (HTS): Serves as a key reagent in fluorescence-based assays for screening compound libraries for BACE1 inhibitors in drug discovery pipelines.
• Biochemical Assay Development: Provides a standardized component for developing and validating sensitive in vitro assays to measure protease activity.
• Mechanistic Studies: Facilitates detailed studies on the cleavage specificity and catalytic mechanism of secretase enzymes.
• Quality Control for Inhibitors: Acts as a reference standard for potency testing and batch consistency evaluation of candidate therapeutic inhibitors.

Basic Information

Product Name	Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770(667-675)-Lys(Dnp) Amide Ammonium Salt
CAS No.	1802078-32-5
Molecular Formula	C64H86N14O19
Molecular Weight	1331.5 g/mol
Synonyms	BACE1 Substrate, Mca-SEVNLDAEFK(Dnp)-NH2 Ammonium Salt; Fluorogenic BACE1 Substrate; Amyloid Precursor Protein (770) Fragment 667-675, Mca-[Asn670, Leu671]-APP770(667-675)-Lys(Dnp) Amide; SEVNLDAEFK(Dnp) Fluorogenic Peptide; β-Secretase Substrate IV; APPsw Substrate; (7-Methoxycoumarin-4-yl)acetyl-SEVNLDAEFK(2,4-Dinitrophenyl)-NH2
EINECS	Contact for details

Quality Control

Every batch of our Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770(667-675)-Lys(Dnp) Amide Ammonium Salt is manufactured under strict quality management systems. We guarantee high purity and batch-to-batch consistency through comprehensive analytical testing, including reverse-phase HPLC, mass spectrometry (MS), and amino acid analysis. A detailed Certificate of Analysis (COA) providing purity, peptide content, and performance data in functional assays is supplied with each shipment to ensure it meets your research specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Reported (Km/Vmax provided on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.