Description

Sulfo-Cy5-Tetrazine is a high-performance, water-soluble fluorescent dye conjugate engineered for advanced bioorthogonal labeling applications. This compound is specifically designed to enable rapid, specific, and catalyst-free conjugation with trans-cyclooctene (TCO) or norbornene-modified biomolecules via the inverse electron-demand Diels-Alder (IEDDA) reaction. It is an essential tool for researchers and developers in the fields of bioconjugation, live-cell imaging, and in vivo diagnostics, offering superior brightness and stability for sensitive detection. The product is supplied under CAS No. 1801695-57-7.

Application

• Bioorthogonal Labeling & Imaging: For specific, rapid tagging of TCO-modified proteins, antibodies, or oligonucleotides in live cells and tissues.
• Pre-Targeted Imaging: Enables multi-step, pre-targeted strategies for in vivo tumor imaging and diagnostics.
• Flow Cytometry & Microscopy: Used as a bright, photostable far-red fluorescent probe for high-resolution cellular analysis.
• Diagnostic Assay Development: Serves as a key component in developing sensitive immunoassays and lateral flow tests.
• Chemical Biology Probes: For tracking biomolecule localization, interaction, and turnover in complex biological systems.
• Nanoparticle & Drug Conjugate Functionalization: For attaching targeting ligands or imaging moieties to delivery platforms.

Basic Information

Product Name	Sulfo-Cy5-Tetrazine
CAS No.	1801695-57-7
Molecular Formula	C44H48N6O10S32- • 2X+ (X = counter ion)
Molecular Weight	~917.1 g/mol (Free acid form)
Synonyms	Sulfo-Cyanine5-Tetrazine; Cy5-sulfo-Tetrazine; Sulfo-Cy5-Tz; Tetrazine-Cy5 sulfonated; 3-(4-(6-(4-(1,2,4,5-Tetrazin-3-yl)benzyl)-1,4-diazepan-1-yl)-4-oxobutyl)-2-((1E,3E)-5-((E)-1,3-dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene)penta-1,3-dien-1-yl)-3-ethyl-1-(4-sulfobutyl)-3H-indol-1-ium; Water-soluble Cy5-Tetrazine
EINECS	Contact for details

Quality Control

Our Sulfo-Cy5-Tetrazine is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for optical property validation. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and functional performance is provided with every shipment to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Dark green to blue solid or lyophilized powder
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λabs)	~646 nm ± 5 nm
Emission Maximum (λem)	~662 nm ± 5 nm
Extinction Coefficient (ε)	≥ 250,000 M-1cm-1
Solubility	Soluble in water, PBS, DMSO
Functional Activity (IEDDA)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.