Description

Tamra-Azide-Peg-Biotin is a multifunctional chemical reagent that combines the fluorescent properties of TAMRA, the bioorthogonal reactivity of an azide group, the solubility and flexibility of a polyethylene glycol (PEG) spacer, and the high-affinity binding of biotin. This compound is engineered for advanced bioconjugation and labeling strategies, enabling precise detection and isolation of biomolecules. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and proteomics research.

Application

• Fluorescent Labeling of Biomolecules: Used to tag proteins, antibodies, or oligonucleotides with the TAMRA fluorophore for visualization in fluorescence microscopy, flow cytometry, or in vivo imaging.
• Click Chemistry Conjugation: The azide group allows for specific, copper-catalyzed or strain-promoted cycloaddition with alkyne-modified targets, facilitating site-specific bioconjugation.
• Affinity Purification and Pull-Down Assays: The biotin moiety enables strong binding to streptavidin/avidin-coated beads or surfaces for the isolation and enrichment of target complexes.
• Development of Diagnostic Probes: Serves as a key component in constructing sensitive probes for ELISA, lateral flow assays, or other biosensing platforms.
• Cell Surface Engineering: Employed to label cell surface proteins or glycans for tracking cell interactions, migration, and signaling events.
• Drug Delivery System Research: The PEG linker improves solubility and can be used to create biotinylated drug conjugates for targeted delivery studies.

Basic Information

Product Name	Tamra-Azide-Peg-Biotin
CAS No.	1797415-74-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-TAMRA-PEG4-Azide-Biotin; TAMRA Azide PEG Biotin; Tetramethylrhodamine-PEG4-Azide-Biotin; 5-Carboxytetramethylrhodamine-PEG4-N3-Biotin; Biotin-PEG4-Azide-5-TAMRA; Biotinylated TAMRA Azide; N3-PEG4-Biotin-TAMRA; 6-(Biotin-PEG4-azido)-5-carboxy-X-rhodamine
EINECS	Contact for details

Quality Control

Our Tamra-Azide-Peg-Biotin is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water
Storage Temperature	-20°C or below

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.