Description

5(6)-Fam-(Glu1)-Orexin A (Human, Mouse, Rat) is a fluorescently labeled analog of the neuropeptide Orexin A, designed for advanced research applications. This compound is critical for studying the orexinergic system, which plays a key role in regulating sleep-wake cycles, arousal, and energy homeostasis. It is an essential tool for researchers in neuroscience, pharmacology, and drug discovery who require high-purity, reliable reagents for receptor binding assays, fluorescence microscopy, and cellular signaling studies.

Application

• Receptor Binding and Competition Assays: Used as a fluorescent tracer to study the binding affinity and kinetics of the orexin receptor OX1R.
• Fluorescence Microscopy and Imaging: Enables visualization and tracking of orexin receptor localization and internalization in live or fixed cells.
• High-Throughput Screening (HTS): Serves as a key reagent in screening campaigns to identify novel agonists or antagonists for orexin receptors.
• Cellular Signaling Pathway Analysis: Facilitates the study of downstream signaling events following receptor activation.
• Neuroscience Research: A vital tool for investigating the role of the orexin system in sleep disorders, narcolepsy, and metabolic regulation.
• Biomarker Development: Potential use in developing diagnostic assays related to orexin pathway dysregulation.

Basic Information

Item	Detail
Product Name	5(6)-Fam-(Glu1)-Orexin A (Human, Mouse, Rat)
CAS No.	1678416-64-2
Molecular Formula	C178H263N51O53S
Molecular Weight	~3950.4 g/mol
Synonyms	FAM-Orexin A; 5(6)-Carboxyfluorescein-Orexin A; Fluorescein-labeled Orexin A; Orexin A (human, mouse, rat), 5(6)-FAM-(Glu(1))-; Orexin A, FAM-labeled; Hypocretin-1, FAM conjugate; OX-A, FAM; Fluorescent Orexin A Peptide
EINECS	Contact for details

Quality Control

Every batch of 5(6)-Fam-(Glu1)-Orexin A is manufactured and tested under strict quality management systems. We guarantee high purity and batch-to-batch consistency through rigorous analytical controls, including reverse-phase HPLC and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and peptide content is provided with each shipment to ensure it meets the exacting standards required for academic and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Absorption Maximum	~494 nm (characteristic of FAM fluorophore)
Solubility	Soluble in water, DMSO, or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.