Description

5-Fam-Hiv-1 Tat Protein (47-57) Trifluoroacetate Salt is a high-purity, fluorescently labeled peptide fragment derived from the HIV-1 Tat protein, specifically the 47-57 sequence, with a 5-FAM (5-Carboxyfluorescein) tag. This compound is a critical research tool for studying protein-protein interactions, cellular uptake mechanisms, and intracellular trafficking, particularly for investigating the cell-penetrating properties of the Tat peptide. It is essential for scientists in pharmaceutical R&D, biotechnology, and academic research focused on virology, drug delivery systems, and peptide-based therapeutics.

Application

• Biomedical Research: Used as a fluorescent probe to study the cell-penetrating peptide (CPP) activity and nuclear localization of the HIV-1 Tat protein domain.
• Drug Delivery Studies: Serves as a model compound for developing and evaluating novel peptide-based drug delivery vectors and cargo transport mechanisms.
• Virology & Immunology: Facilitates research into HIV-1 pathogenesis, Tat protein function, and host-pathogen interactions.
• Fluorescence Microscopy & Imaging: The 5-FAM label enables direct visualization and tracking of peptide uptake, distribution, and localization within live or fixed cells.
• Binding Assays: Employed in fluorescence polarization (FP), fluorescence resonance energy transfer (FRET), and other biophysical assays to quantify molecular interactions.
• Peptide Therapeutics Development: Acts as a key intermediate or reference standard in the design and optimization of therapeutic peptides targeting intracellular processes.

Basic Information

Product Name	5-Fam-Hiv-1 Tat Protein (47-57) Trifluoroacetate Salt
CAS No.	1676104-81-6
Molecular Formula	C78H101N19O22 • xC2HF3O2
Molecular Weight	~1680.8 g/mol (Free base)
Synonyms	5-FAM-Tat(47-57); 5-Carboxyfluorescein-Tat(47-57); FAM-labeled HIV-1 Tat peptide (47-57); GRKKRRQRRRPP(5-FAM); HIV-1 Trans-Activator of Transcription Peptide (47-57), 5-FAM Labeled; TFA salt of 5-FAM-Tat(47-57); Fluorescent Tat Cell-Penetrating Peptide
EINECS	Contact for details

Quality Control

Our 5-Fam-Hiv-1 Tat Protein (47-57) Trifluoroacetate Salt is manufactured under strict quality systems suitable for research use. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. Certificates of Analysis (COA) are provided, detailing results from HPLC, MS, and other relevant analyses. We support compliance with ISO 9001 standards and can provide documentation for research and development purposes.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70%
Amino Acid Analysis	Within ± 10% of theoretical
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 8.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.