Description

Methyltetrazine-Nhs Ester CAS NO 1644644-96-1 is a highly reactive and versatile heterobifunctional crosslinking agent, combining a methyltetrazine moiety with an N-hydroxysuccinimide (NHS) ester. This compound is specifically engineered for efficient and selective bioconjugation, enabling the stable labeling of biomolecules under mild, aqueous conditions. It is an essential reagent for researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science, where precise and robust chemical linkages are required.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via bioorthogonal chemistry.
• Fluorescent Probe & Diagnostic Reagent Synthesis: Labeling proteins, peptides, and oligonucleotides with fluorophores or other reporter molecules for imaging and detection.
• Polymer & Biomaterial Functionalization: Introducing bio-recognition elements onto surfaces, nanoparticles, or hydrogel matrices for targeted drug delivery or biosensing.
• Chemical Biology & Proteomics Research: As a key tool for studying protein-protein interactions, cellular trafficking, and enzyme activity through bioorthogonal labeling strategies.
• Peptide & Protein Modification: Facilitating the covalent attachment of various functional groups to amine-containing biomolecules.
• Next-Generation Sequencing (NGS) Library Preparation: For tagging nucleic acids in advanced sequencing workflows.

Basic Information

Product Name	Methyltetrazine-Nhs Ester
CAS No.	1644644-96-1
Molecular Formula	C14H14N6O4
Molecular Weight	330.30 g/mol
Synonyms	5-Methyl-1,2,4,5-tetrazin-3(2H)-one NHS ester; 3-(5-Methyl-1,2,4,5-tetrazin-3-yl)propanoic acid N-hydroxysuccinimide ester; Methyltetrazine-PEG3-NHS ester; Methyl-Tz-NHS; 5-Methyltetrazine NHS Ester; Tetrazine-NHS crosslinker; Bioorthogonal NHS ester reagent
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Nhs Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We adhere to cGMP principles where applicable to support our customers in regulated research and development environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.