Description

(Lys(Me)2)-Histone H3 (1-21)-Gly-Gly-Lys(Biotinyl) CAS NO 1622085-04-4 is a chemically modified, biotinylated peptide fragment derived from the N-terminal tail of histone H3, specifically engineered for advanced epigenetic and proteomic research. This compound is a critical reagent for studying post-translational modifications, particularly dimethylation of lysine residues, and their role in chromatin structure and gene regulation. It is primarily utilized by researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on epigenetics, biomarker discovery, and assay development.

Application

• Epigenetic Research: As a substrate or standard for investigating histone methylation dynamics, enzyme activity (e.g., methyltransferases, demethylases), and reader domain interactions.
• Biomarker Development: Utilized in the discovery and validation of epigenetic biomarkers associated with various diseases, including oncology.
• Diagnostic Assay Development: Serves as a key component in ELISA, western blot, or other immunoassay platforms for detecting specific histone modifications.
• Drug Discovery Screening: Used in high-throughput screening (HTS) assays to identify and characterize potential therapeutic compounds that modulate epigenetic targets.
• Pull-down and Affinity Purification: The biotin tag enables efficient streptavidin-based capture of interacting proteins or complexes for mass spectrometric analysis (e.g., ChIP-seq related workflows).
• Antibody Specificity Testing: Acts as a critical positive control for validating the specificity of antibodies raised against dimethylated lysine residues on histone H3.
• Peptide Array and Microarray Fabrication: Used in constructing peptide arrays to profile antibody or protein binding specificity on a large scale.

Basic Information

Product Name	(Lys(Me)2)-Histone H3 (1-21)-Gly-Gly-Lys(Biotinyl)
CAS No.	1622085-04-4
Molecular Formula	C136H231N43O38S
Molecular Weight	3088.6 g/mol (theoretical)
Synonyms	H3(1-21) peptide, dimethyl Lys4, biotinylated; Biotinyl-Histone H3 (1-21), Lys(Me)2; ARTKQTARK(Me)2STGGKAPRKQLA-GG-K(Biotin); Histone H3 (1-21) (dimethyl Lys4) Biotin conjugate; Epigenetic Peptide Substrate H3K4me2-Biotin; Biotinylated H3K4me2 Peptide; N-terminal Histone H3 Peptide, Biotinylated and Dimethylated; Ac-ARTKQTARK(Me2)STGGKAPRKQLA-GG-K(Biotin)-NH2
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of (Lys(Me)2)-Histone H3 (1-21)-Gly-Gly-Lys(Biotinyl) is characterized using advanced analytical techniques to guarantee identity, purity, and correct modification. Certificates of Analysis (COA) detailing HPLC purity, MS (Mass Spectrometry) confirmation, and correct amino acid composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption. Aliquot into single-use quantities to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical composition
Modification Verification	Correct dimethylation at Lys4 and biotinylation confirmed by MS/MS
Solubility	Soluble in water or aqueous buffers; consult TDS for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.