Description

Tco-Peg4-Nhs Ester CAS NO 1613439-69-2 is a heterobifunctional crosslinking reagent featuring a trans-cyclooctene (TCO) group for bioorthogonal click chemistry and an N-hydroxysuccinimide (NHS) ester for efficient amine coupling. This compound is essential for creating stable, site-specific bioconjugates under mild, physiological conditions without the need for toxic catalysts. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, proteomics, and in vivo imaging for labeling and modifying biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Linker Synthesis: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies for targeted cancer therapies.
• Protein and Peptide Labeling: Functionalization of amine-containing proteins, peptides, and enzymes with TCO tags for subsequent tetrazine-based click reactions.
• Surface Modification and Immobilization: Crafting functionalized surfaces (e.g., biosensors, microarrays) by conjugating TCO ligands to amine-coated substrates.
• In Vivo Pretargeting Strategies: Enabling multi-step pretargeting approaches in radioimmunotherapy and diagnostic imaging for improved target-to-background ratios.
• Polymer and Nanoparticle Functionalization: Introducing bioorthogonal handles onto amine-functionalized polymers, dendrimers, or nanoparticles for advanced drug delivery systems.
• Fluorescent Probe and Diagnostic Reagent Development: Creating turn-key reagents for sensitive detection assays via rapid tetrazine ligation.

Basic Information

Product Name	Tco-Peg4-Nhs Ester
CAS No.	1613439-69-2
Molecular Formula	C27H43N3O9
Molecular Weight	553.65 g/mol
Synonyms	trans-Cyclooctene-PEG4-NHS Ester; TCO-PEG4-NHS; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12-tetraoxapentadecan-15-yl) carbamate; TCO-PEG4-NHS Ester; TCO-PEG4-NHS Ester; TCO-PEG4-NHS; TCO-PEG4-N-hydroxy succinimide ester; TCO-PEG4-SCM
EINECS	Contact for details

Quality Control

Our Tco-Peg4-Nhs Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.