Description

Dbco-Nh-Boc is a high-purity, bifunctional chemical linker featuring a strained alkyne (DBCO) for bioorthogonal click chemistry and a protected amine (Boc-NH) for subsequent conjugation. This compound matters for its role in facilitating controlled, multi-step bioconjugation strategies, enabling the precise assembly of complex biomolecular architectures. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and advanced material science who require reliable, site-specific labeling and crosslinking agents.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Site-specific coupling of payloads, dyes, or peptides to biomolecules via the DBCO group, with the Boc-protected amine allowing for orthogonal deprotection and further functionalization.
• Proteomics and Biomarker Discovery: Labeling of proteins or glycans for mass spectrometry analysis, fluorescence imaging, or pull-down assays.
• Polymer and Material Science: Synthesis of functionalized hydrogels, surfaces, or nanoparticles with bioactive ligands for cell culture or diagnostic applications.
• Chemical Biology Tool Development: Creation of activity-based probes or affinity tags to study enzyme function or protein-protein interactions.
• Peptide and Oligonucleotide Synthesis: As a building block for introducing clickable handles and protected amine functionalities into synthetic chains.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens on sensor surfaces for ELISA or lateral flow assays.

Basic Information

Product Name	Dbco-Nh-Boc
CAS No.	1539290-74-8
Molecular Formula	C24H24N2O4
Molecular Weight	404.46 g/mol
Synonyms	DBCO-NH-Boc; DBCO-Amine-Boc; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-(tert-butoxycarbonyl)carbamate; N-Boc-1,8-diazacyclooct-4-yne-1-carboxamide; Dibenzocyclooctyne-PEG4-amine-Boc; DBCO-PEG4-NH-Boc; tert-Butyl (DBCO-PEG4)carbamate; BCN-PEG4-NH-Boc
EINECS	Contact for details

Quality Control

Every batch of Dbco-Nh-Boc is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR, and mass spectrometry. Our quality commitment aligns with the standards required for research and cGMP-grade applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.