Description

Cy7.5 NHS Ester is a near-infrared (NIR) fluorescent labeling reagent designed for the covalent conjugation of biomolecules via primary amines. This compound is critical for researchers and developers requiring stable, high-performance fluorescent tags for sensitive detection and imaging applications. It is essential for professionals in bioconjugation, bioassay development, and in vivo imaging across the pharmaceutical, diagnostic, and life science research sectors.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, proteins, and peptides for fluorescence-activated cell sorting (FACS), immunofluorescence, and Western blotting.
• Nucleic Acid Probe Development: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• In Vivo Imaging Agent Synthesis: Creation of targeted NIR probes for deep-tissue imaging in preclinical small animal studies.
• Diagnostic Assay Manufacturing: Production of fluorescent conjugates for lateral flow assays, ELISA, and other clinical diagnostic kits.
• Nanoparticle Functionalization: Surface modification of polymeric or silica nanoparticles for advanced drug delivery and imaging systems.
• Biomaterial and Polymer Labeling: Tagging of hydrogels, scaffolds, and other polymeric materials to track degradation or cell interaction.

Basic Information

Product Name	Cy7.5 NHS Ester
CAS No.	1469278-04-3
Molecular Formula	C45H51ClN2O5
Molecular Weight	735.35 g/mol
Synonyms	Cy7.5 N-Hydroxysuccinimide Ester; Sulfo-Cyanine7.5 NHS Ester; 1-(6-((E)-2-((E)-2-Chloro-3-((E)-2-(1-(2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3-sulfopropan-2-yl)amino)ethylidene)-3,3-dimethyl-5-sulfo-1-((E)-2-(1,1,7,7-tetramethyl-2,3,6,7-tetrahydro-1H,5H-pyrano[3,2-f]quinolin-9-yl)vinyl)indolin-1-ium-2-yl)vinyl)-1,1,7,7-tetramethyl-2,3,6,7-tetrahydro-1H,5H-pyrano[3,2-f]quinolin-9-ium; Cyanine7.5 NHS Ester; NIR-797 NHS Ester (related analog); Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our Cy7.5 NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation work. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and moisture content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	788 ± 5 nm
Emission Maximum (λem)	808 ± 5 nm
Extinction Coefficient (ε)	≥ 200,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.