Description

Biotin-Peg3-Mal is a heterobifunctional crosslinker featuring a biotin group for affinity capture and a maleimide (Mal) moiety for site-specific conjugation to thiols (cysteine residues), connected by a triethylene glycol (PEG3) spacer. This compound is essential for creating stable, site-specific bioconjugates with enhanced solubility and reduced steric hindrance, critical for advanced biochemical assays. It is primarily utilized by researchers and manufacturers in biopharmaceutical development, diagnostic kit production, and proteomics research for labeling proteins, antibodies, and other biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via engineered cysteine residues.
• Diagnostic Assay Development: Biotinylation of detection antibodies or proteins for use in ELISA, lateral flow assays, and other immunoassay platforms.
• Affinity Purification Probes: Immobilization of thiol-containing ligands or capture agents onto streptavidin/avidin-coated resins or surfaces.
• Protein and Peptide Labeling: Conjugation of biotin tags to cysteine-containing proteins or synthetic peptides for detection, pull-down, or imaging studies.
• Surface Functionalization: Modification of gold nanoparticles or other thiol-reactive surfaces with biotin for biosensor and nanotechnology applications.
• Structural Biology: Introduction of biotin handles into proteins for cryo-EM sample preparation or other structural analysis techniques.

Basic Information

Product Name	Biotin-Peg3-Mal
CAS No.	1431618-70-0
Molecular Formula	C₂₄H₃₉N₅O₈S
Molecular Weight	565.66 g/mol
Synonyms	Biotin-PEG3-maleimide; Biotin-PEG3-Mal; Maleimide-PEG3-Biotin; 3-((6-((2-(2-(3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanamido)ethoxy)ethoxy)ethyl)amino)-6-oxohexyl)carbamoyl)-5-(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; Biotin-triethyleneglycol-maleimide; Biotin-PEG3-Maleimide Crosslinker; EZ-Link Biotin-PEG3-Maleimide
EINECS	Contact for details

Quality Control

Our Biotin-Peg3-Mal is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science research and bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the maleimide group.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.