Description

Dbco-S-S-Peg3-Biotin is a heterobifunctional crosslinking reagent that combines a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) with a biotin moiety for streptavidin/avidin capture, connected via a cleavable disulfide (S-S) bond and a triethylene glycol (PEG3) spacer. This compound is specifically engineered for advanced bioconjugation strategies, enabling the efficient, copper-free labeling of biomolecules followed by subsequent purification or detection, with the added capability for controlled release under reducing conditions. It is an essential tool for researchers and developers in biopharmaceuticals, diagnostic assay development, and proteomics who require precise, efficient, and reversible site-specific modifications.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via SPAAC click chemistry, with a cleavable linker for intracellular drug release.
• Diagnostic Probe & Biosensor Fabrication: Used to immobilize and orient biomolecules (e.g., antibodies, peptides) on sensor surfaces or nanoparticles for enhanced assay sensitivity and specificity.
• Affinity Purification & Pull-Down Assays: Facilitates the biotinylation of target proteins or nucleic acids for capture and isolation using streptavidin beads, with the disulfide bond allowing for gentle elution.
• Cell Surface Labeling & Imaging: Enables copper-free click chemistry labeling of azide-modified glycans or other cell surface structures for live-cell imaging and tracking studies.
• Protein & Peptide Modification: A versatile reagent for introducing biotin tags onto azide-functionalized proteins or peptides for detection, quantification, or interaction studies.
• Polymer & Material Science: Serves as a functional linker for modifying polymers or nanomaterials with biotin groups to create smart, responsive materials for biomedical applications.

Basic Information

Product Name	Dbco-S-S-Peg3-Biotin
CAS No.	1430408-09-5
Molecular Formula	C₄₀H₅₄N₄O₉S₃
Molecular Weight	831.08 g/mol
Synonyms	DBCO-S-S-PEG3-Biotin; DBCO-PEG3-SS-Biotin; Dibenzocyclooctyne-PEG3-disulfide-biotin; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2-{2-[2-({2-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]ethyl}disulfanyl)ethoxy]ethoxy}ethyl)carbamate; DBCO-SS-PEG3-Biotin; S-S-DBCO-PEG3-Biotin; DBCO-C3-SS-Biotin; Azadibenzocyclooctyne-PEG3-disulfide-biotin conjugate
EINECS	Contact for details

Quality Control

Our Dbco-S-S-Peg3-Biotin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, NMR and MS for structural confirmation, and functional assay validation to ensure optimal performance in click chemistry applications. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are provided and available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (SPAAC)	≥ 90% conjugation efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.