Description

n-Succinimidyl 4-(Azidosulfonyl)Benzoate is a specialized heterobifunctional crosslinking reagent designed for the efficient conjugation of biomolecules. Its core value lies in its dual-reactive functionality, featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable aryl azide group, enabling controlled, light-induced crosslinking. This compound is essential for researchers and manufacturers in bioconjugation, proteomics, and antibody-drug conjugate (ADC) development, where precise and stable linkage formation is critical.

Application

• Bioconjugation and Protein Labeling: For covalently attaching labels, drugs, or other molecules to proteins, antibodies, and peptides via amine groups.
• Surface Immobilization: Used to functionalize biosensor chips, microarray surfaces, or nanoparticles with biomolecules for diagnostic and research applications.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key chemical building block in the synthesis of cleavable or non-cleavable linkers for targeted cancer therapies.
• Photoaffinity Labeling: The aryl azide group allows for the capture and identification of protein-protein interactions or ligand-receptor binding sites upon UV light activation.
• Materials Science: Incorporation into polymer backbones or hydrogels to create photo-crosslinkable, biofunctional materials.
• Diagnostic Reagent Synthesis: Manufacturing of enzyme conjugates or fluorescent probes for immunoassays (ELISA, lateral flow tests).

Basic Information

Product Name	n-Succinimidyl 4-(Azidosulfonyl)Benzoate
CAS No.	1427502-22-4
Molecular Formula	C11H8N4O6S
Molecular Weight	324.27 g/mol
Synonyms	4-(Azidosulfonyl)benzoic acid N-succinimidyl ester; NHS-ASA; N-Succinimidyl 4-azidosulfonylbenzoate; SASD; Sulfosuccinimidyl 2-([4-azidosalicylamido]ethyl-1,3'-dithiopropionate (related compound); 4-Azidosulfonylbenzoic Acid Succinimidyl Ester; Heterobifunctional Crosslinker SASD; Photoactivatable NHS Ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 4-(Azidosulfonyl)Benzoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high reactivity. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and FT-IR for functional group verification. Certificates of Analysis (COA) detailing all specifications are provided to support your research, development, and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.